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. 1967 May;93(5):1479–1483. doi: 10.1128/jb.93.5.1479-1483.1967

Physiology of Rickettsiae VII. Amino Acid Incorporation by Coxiella burnetii and by Infected Hosts

L P Mallavia a,1, D Paretsky a
PMCID: PMC276636  PMID: 6025436

Abstract

Protein synthesis, in terms of 14C-labeled amino acid incorporation into a hot trichloroacetic acid fraction, was studied in cell-free preparations of Coxiella burnetii, and in uninfected and Q fever-infected guinea pig and chick embryo hosts. Purified and disrupted suspensions of C. burnetii incorporated 14C-labeled l-leucine, l-phenylalanine and algal hydrolysate. Livers of infected guinea pigs and chick embryos had a greater incorporation rate at the height of infection than comparable preparations from uninfected animals. As chick embryonic development continued during infection, the rate of incorporation progressively decreased below that of uninfected embryos.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOVARNICK M. R., SCHNEIDER L. The incorporation of glycine-1-C14 by typhus rickettsiae. J Biol Chem. 1960 Jun;235:1727–1731. [PubMed] [Google Scholar]
  2. BOVARNICK M. R., SCHNEIDER L., WALTER H. The incorporation of labeled methionine by typhus rickettsiae. Biochim Biophys Acta. 1959 Jun;33(2):414–422. doi: 10.1016/0006-3002(59)90131-3. [DOI] [PubMed] [Google Scholar]
  3. CONSIGLI R. A., PARETSKY D. Oxidation of glucose 6-phosphate and isocitrate by Coxiella burnetii. J Bacteriol. 1962 Jan;83:206–207. doi: 10.1128/jb.83.1.206-207.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FUJITA K., KOHNO S., SHISHIDO A. The incorporation of methionine into purified rickettsiae. Jpn J Med Sci Biol. 1959 Oct;12:387–390. doi: 10.7883/yoken1952.12.387. [DOI] [PubMed] [Google Scholar]
  5. FULTON J. D., GRANT P. T. The sulphur requirements of the erythrocytic from of Plasmodium knowlesi. Biochem J. 1956 Jun;63(2):274–282. doi: 10.1042/bj0630274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GAETANI S., PAOLUCCI A. M., SPADONI M. A., TOMASSI G. ACTIVITY OF AMINO ACID-ACTIVATING ENZYMES IN TISSUES FROM PROTEIN-DEPLETED RATS. J Nutr. 1964 Oct;84:173–178. doi: 10.1093/jn/84.2.173. [DOI] [PubMed] [Google Scholar]
  7. GLUCK L., KULOVICH M. V. NUCLEIC ACID AND PROTEIN CHANGES DURING EMBRYOMIC ORGAN DEVELOPMENT IN THE CHICK. Yale J Biol Med. 1964 Apr;36:361–378. [PMC free article] [PubMed] [Google Scholar]
  8. GREIFF D. The metabolic interactions of intracellular parasites and embryonate eggs. Ann N Y Acad Sci. 1952 Aug 8;55(2):254–266. doi: 10.1111/j.1749-6632.1952.tb26541.x. [DOI] [PubMed] [Google Scholar]
  9. Jones F., Jr, Paretsky D. Physiology of rickettsiae. VI. Host-independent synthesis of polyribonucleotides by Coxiella burnetii. J Bacteriol. 1967 Mar;93(3):1063–1068. doi: 10.1128/jb.93.3.1063-1068.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KOHNO S., NATSUME Y., SHISHIDO A. Studies on metabolism of rickettsiae. II. Incorporation of 14C amino acid mixture and 35S methionine by Rickettsia mooseri. Jpn J Med Sci Biol. 1961 Dec;14:213–222. doi: 10.7883/yoken1952.14.213. [DOI] [PubMed] [Google Scholar]
  11. KORNER A. The role of the adrenal gland in the control of amino acid incorporation into protein of isolated rat liver microsomes. J Endocrinol. 1960 Nov;21:177–189. doi: 10.1677/joe.0.0210177. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MALLAVIA L., PARETSKY D. STUDIES ON THE PHYSIOLOGY OF RICKETTSIAE. V. METABOLISM OF CARBAMYL PHOSPHATE BY COXIELLA BURNETII. J Bacteriol. 1963 Aug;86:232–238. doi: 10.1128/jb.86.2.232-238.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MYERS W. F., PARETSKY D. Synthesis of serine by Coxiella burnetii. J Bacteriol. 1961 Nov;82:761–763. doi: 10.1128/jb.82.5.761-763.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PARETSKY D., CONSIGLI R. A., DOWNS C. M. Studies on the physiology of rickettsiae. III. Glucose phosphorylation and hexokinase activity in Coxiella burnetii. J Bacteriol. 1962 Mar;83:538–543. doi: 10.1128/jb.83.3.538-543.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PARETSKY D., DOWNS C. M., CONSIGLI R. A., JOYCE B. K. Studies on the physiology of rickettsiae. I. Some enzyme systems of Coxiella burnetii. J Infect Dis. 1958 Jul-Aug;103(1):6–11. doi: 10.1093/infdis/103.1.6. [DOI] [PubMed] [Google Scholar]
  17. PARETSKY D., DOWNS C. M., SALMON C. W. SOME BIOCHEMICAL CHANGES IN THE GUINEA PIG DURING INFECTION WITH COXIELLA BURNETII. J Bacteriol. 1964 Jul;88:137–142. doi: 10.1128/jb.88.1.137-142.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SIEKEVITZ P. Uptake of radioactive alanine in vitro into the proteins of rat liver fractions. J Biol Chem. 1952 Apr;195(2):549–565. [PubMed] [Google Scholar]
  19. Schweet R., Lamfrom H., Allen E. THE SYNTHESIS OF HEMOGLOBIN IN A CELL-FREE SYSTEM. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1029–1035. doi: 10.1073/pnas.44.10.1029. [DOI] [PMC free article] [PubMed] [Google Scholar]

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