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. 1967 May;93(5):1598–1606. doi: 10.1128/jb.93.5.1598-1606.1967

Growth of Pathogenic Leptospira in Chemically Defined Media1

Esther Shenberg a
PMCID: PMC276655  PMID: 6025446

Abstract

A protein-free chemically defined medium for cultivation of pathogenic Leptospira was developed. The medium permitted continued serial subculturing of 9 serogroups (52 strains) of the 12 serogroups (61 strains) tested. Growth was initiated from small inocula, and the growth rate and maximal cell yields were similar to those on serum-containing media. The nutritional requirements of serogroups L. canicola, L. pomona, and L. grippotyphosa were studied in a basal medium composed of inorganic salts, a fatty acid, vitamin B12, and thiamine. All strains tested utilized ammonium chloride as the sole nitrogen source. A fatty acid, vitamin B12, and ferrous ions were essential. Growth was stimulated by thiamine, potassium, and calcium ions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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