Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Jun;93(6):1853–1862. doi: 10.1128/jb.93.6.1853-1862.1967

Phospholipids and Glycolipids of Sterol-requiring Mycoplasma

Paul F Smith 1, Walter L Koostra 1
PMCID: PMC276702  PMID: 6025304

Abstract

The phospholipids of Mycoplasma hominis type 2 strain 07 are composed almost entirely of phosphatidyl glycerol. Traces of other glycerophospholipids may exist. No glycolipids are found. The phospholipids of Mycoplasma sp. avian strain J are composed of diphosphatidyl glycerol, which predominates in older cultures, a monoacyl glycerophosphoryl glycerophosphate, which may serve as a precursor of diphosphatidyl glycerol, and phosphatidyl glycerophosphate. This organism also contains cholesteryl glucoside and an unidentified glycolipid which appears to be similar to a monoglucosyl diglyceride. No turnover or radioisotope labeling of the phospholipids occurs during metabolism. This lack of turnover during growth is indicative of a structural role for these glycerophospholipids. A concomitant decrease of monoacyl glycerophosphoryl glycerophosphate and increase of diphosphatidyl glycerol occurs during growth.

Full text

PDF
1853

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUNDISH D. E., SHAW N., BADDILEY J. THE OCCURRENCE OF GLYCOLIPIDS IN GRAM-POSITIVE BACTERIA. Biochem J. 1965 May;95:21C–22C. doi: 10.1042/bj0950021c. [DOI] [PubMed] [Google Scholar]
  2. Burkholder P. R., McVeigh I., Moyer D. Studies on Some Growth Factors of Yeasts. J Bacteriol. 1944 Oct;48(4):385–391. doi: 10.1128/jb.48.4.385-391.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. Freeman C. P., West D. Complete separation of lipid classes on a single thin-layer plate. J Lipid Res. 1966 Mar;7(2):324–327. [PubMed] [Google Scholar]
  5. HANAHAN D. J., BROCKERHOFF H., BARRON E. J. The site of attack of phospholipase (lecithinase) A on lecithin: a re-evaluation. Position of fatty acids on lecithins and triglycerides. J Biol Chem. 1960 Jul;235:1917–1923. [PubMed] [Google Scholar]
  6. HANAHAN D. J., OLLEY J. N. Chemical nature of monophosphoinositides. J Biol Chem. 1958 Apr;231(2):813–828. [PubMed] [Google Scholar]
  7. HOUTSMULLER U. M., van DEENEN L. Identification of a bacterial phospholipid as an O-ornithine ester of phosphatidyl glycerol. Biochim Biophys Acta. 1963 Apr 23;70:211–213. doi: 10.1016/0006-3002(63)90743-1. [DOI] [PubMed] [Google Scholar]
  8. HULLEY S. B., JORGENSEN S. B., LIN E. C. Ribitol dehydrogenase in Aerobacter aerogenes 1033. Biochim Biophys Acta. 1963 Feb 12;67:219–225. doi: 10.1016/0006-3002(63)91819-5. [DOI] [PubMed] [Google Scholar]
  9. Henrikson C. V., Smith P. F. Conversion of mevalonic acid to gamma, gamma-dimethylallyl pyrophosphate by Mycoplasma. J Bacteriol. 1966 Sep;92(3):701–706. doi: 10.1128/jb.92.3.701-706.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Houtsmuller U. M., van Deenen L. L. On the amino acid esters of phosphatidyl glycerol from bacteria. Biochim Biophys Acta. 1965 Dec 2;106(3):564–576. doi: 10.1016/0005-2760(65)90072-x. [DOI] [PubMed] [Google Scholar]
  11. Ikawa M., Morrow J. W., Harney S. J. Paper chromatographic system for the identification of glycerol in bacterial cell walls. J Bacteriol. 1966 Oct;92(4):812–814. doi: 10.1128/jb.92.4.812-814.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
  13. MALPRESS F. H., MORRISON A. B. Use of pyridine in the de-ionization of solutions for paper chromatography. Nature. 1949 Dec 3;164(4179):963–963. doi: 10.1038/164963a0. [DOI] [PubMed] [Google Scholar]
  14. PARK J. T., JOHNSON M. J. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed] [Google Scholar]
  15. RAPPORT M. M., ALONZO N. Photometric determination of fatty acid ester groups in phospholipides. J Biol Chem. 1955 Nov;217(1):193–198. [PubMed] [Google Scholar]
  16. ROTHBLAT G. H., SMITH P. F. Nonsaponifiable lipids of representative pleuropneumonia-like organisms. J Bacteriol. 1961 Oct;82:479–491. doi: 10.1128/jb.82.4.479-491.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  19. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]
  20. de Haas G. H., Bonsen P. P., van Deenen L. L. Studies on cardiolipin. 3. Structural identity of ox-heart cardiolipin and synthetic diphosphatidyl glycerol. Biochim Biophys Acta. 1966 Feb 1;116(1):114–124. doi: 10.1016/0005-2760(66)90097-x. [DOI] [PubMed] [Google Scholar]
  21. den Kamp JA O. P., Houtsmuller U. M., van Deenen L. L. On the phospholipids of Bacillus megaterium. Biochim Biophys Acta. 1965 Oct 4;106(2):438–441. doi: 10.1016/0005-2760(65)90059-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES