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. 1967 Jun;93(6):1917–1924. doi: 10.1128/jb.93.6.1917-1924.1967

Thymineless Death in Escherichia coli: Strain Specificity

Donald J Cummings 1, Lee Mondale 1
PMCID: PMC276711  PMID: 5337772

Abstract

Thymineless death of various ultraviolet (UV)-sensitive strains of Escherichia coli B and K-12 was investigated. It was found that E. coli B, Bs−12, K-12 rec-21, and possibly K-12 Lon−, all sensitive to UV, were also sensitive to thymine starvation. However, other UV-sensitive strains of E. coli were found to display the typical resistant-type kinetics of thymineless death. The correlation of these results with various other cellular processes suggested that the filament-forming ability of the bacteria might be involved in the mechanism of thymineless death. It was apparent from the present results that capacity for host-cell reactivation, recombination ability, thymine dimer excision, and probably induction of a defective prophage had little to do with determining sensitivity to thymine deprivation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark A. J., Chamberlin M., Boyce R. P., Howard-Flanders P. Abnormal metabolic response to ultraviolet light of a recombination deficient mutant of Escherichia coli K12. J Mol Biol. 1966 Aug;19(2):442–454. doi: 10.1016/s0022-2836(66)80015-3. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. S., Barner H. D. STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1954 Oct;40(10):885–893. doi: 10.1073/pnas.40.10.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cummings D. J., Taylor A. L. Thymineless death and its relation to UV sensitivity in Escherichia coli. Proc Natl Acad Sci U S A. 1966 Jul;56(1):171–176. doi: 10.1073/pnas.56.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doudney C. O. Requirement for ribonucleic acid synthesis for deoxyribonucleic acid replication in bacteria. Nature. 1966 Jul 2;211(5044):39–41. doi: 10.1038/211039a0. [DOI] [PubMed] [Google Scholar]
  7. FRAMPTON E. W., BRINKLEY B. R. EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:446–452. doi: 10.1128/jb.90.2.446-452.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FRAMPTON E. W. SYNTHESIS OF RIBONUCLEIC ACID BY X-IRRADIATED BACTERIA. J Bacteriol. 1964 Jun;87:1369–1376. doi: 10.1128/jb.87.6.1369-1376.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FREIFELDER D., MAALOE O. ENERGY REQUIREMENT FOR THYMINELESS DEATH IN CELLS OF ESCHERICHIA COLI. J Bacteriol. 1964 Oct;88:987–990. doi: 10.1128/jb.88.4.987-990.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fredericq P. On the nature of colicinogenic factors: a review. J Theor Biol. 1963 Mar;4(2):159–165. doi: 10.1016/0022-5193(63)90024-9. [DOI] [PubMed] [Google Scholar]
  11. GALLANT J., SUSKIND S. R. Ribonucleic acid synthesis and thymineless death. Biochim Biophys Acta. 1962 May 14;55:627–638. doi: 10.1016/0006-3002(62)90841-7. [DOI] [PubMed] [Google Scholar]
  12. HANAWALT P. C. Involvement of synthesis of RNA in thymineless death. Nature. 1963 Apr 20;198:286–286. doi: 10.1038/198286a0. [DOI] [PubMed] [Google Scholar]
  13. HILL R. F., FEINER R. R. FURTHER STUDIES OF ULTRAVIOLET-SENSITIVE MUTANTS OF ESCHERICHIA COLI STRAIN B. J Gen Microbiol. 1964 Apr;35:105–114. doi: 10.1099/00221287-35-1-105. [DOI] [PubMed] [Google Scholar]
  14. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KORN D., WEISSBACH A. Thymineless induction in Escherichia coli K12 (lambda). Biochim Biophys Acta. 1962 Nov 26;61:775–790. doi: 10.1016/0926-6550(62)90060-9. [DOI] [PubMed] [Google Scholar]
  17. LUZZATI D., REVEL C. [Lethal effect of thymine deficiency: state of desoxyribonucleic acid during this deficiency]. Biochim Biophys Acta. 1962 Aug 20;61:305–306. [PubMed] [Google Scholar]
  18. Lieb M. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages. J Mol Biol. 1966 Mar;16(1):149–163. doi: 10.1016/s0022-2836(66)80269-3. [DOI] [PubMed] [Google Scholar]
  19. Luzzati D. Effect of thymine starvation on messenger ribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1966 Nov;92(5):1435–1446. doi: 10.1128/jb.92.5.1435-1446.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. MELECHEN N. E., SKAAR P. D. The provocation of an early step of induction by thymine deprivation. Virology. 1962 Jan;16:21–29. doi: 10.1016/0042-6822(62)90198-8. [DOI] [PubMed] [Google Scholar]
  21. MENNIGMANN H. D., SZYBALSKI W. Molecular mechanism of thymine-less death. Biochem Biophys Res Commun. 1962 Nov 27;9:398–404. doi: 10.1016/0006-291x(62)90023-2. [DOI] [PubMed] [Google Scholar]
  22. Mennigmann H. D. Induction in Escherichia coli 15 of the colicinogenic factor by thymine-less death. Biochem Biophys Res Commun. 1964 Jul 1;16(4):373–378. doi: 10.1016/0006-291x(64)90043-9. [DOI] [PubMed] [Google Scholar]
  23. Mennigmann H. D. Phagen-Indultion, Filament-Bildung und Thymin-Mangel-Tod bei Escherichia coli B. Zentralbl Bakteriol Orig. 1965 Dec;198(1):282–286. [PubMed] [Google Scholar]
  24. NAKADA D., RYAN F. J. Replication of deoxyribonucleic acid in non-dividing bacteria. Nature. 1961 Feb 4;189:398–399. doi: 10.1038/189398a0. [DOI] [PubMed] [Google Scholar]
  25. OKADA T., YANAGISAWA K., RYAN F. J. Elective production of thymine-less mutants. Nature. 1960 Oct 22;188:340–341. doi: 10.1038/188340a0. [DOI] [PubMed] [Google Scholar]
  26. OTSUJI N., SEKIGUCHI M., IIJIMA T., TAKAGI Y. Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Nature. 1959 Oct 3;184(Suppl 14):1079–1080. doi: 10.1038/1841079b0. [DOI] [PubMed] [Google Scholar]
  27. PETTIJOHN D., HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. doi: 10.1016/s0022-2836(64)80216-3. [DOI] [PubMed] [Google Scholar]
  28. Pauling C., Hanawalt P. Nonconservative DNA replication in bacteria after thymine starvation. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1728–1735. doi: 10.1073/pnas.54.6.1728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Setlow R. B., Carrier W. L., Williams R. W. Three Levels of DNA Repair in E. coli. Science. 1966 Apr 29;152(3722):676–676. doi: 10.1126/science.152.3722.676-c. [DOI] [PubMed] [Google Scholar]

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