Abstract
Isolated and purified cell walls of Staphylococcus aureus were treated with a purified fraction of the culture supernatant fluid of a species of Aeromonas. The course of lysis of the cell walls was followed over a period of time by examination of samples under an electron microscope. The undifferentiated cell wall was rapidly digested, but the equatorial rings were more resistant. The undifferentiated cell wall became a very thin sheet before completely dissolving, leaving a series of equatorial rings of various widths. As digestion proceeded, solubilization of the entire cell wall occurred. Analogous findings were obtained with purified S. aureus mucopeptide. It is concluded that the Aeromonas lytic principle is an enzyme, and that susceptible bonds are more concentrated in the undifferentiated cell wall mucopeptide.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Coles N. W., Gilbo C. M. Lysis of Staphylococcus aureus by culture supernatant fluids of a species of Aeromonas. J Bacteriol. 1967 Mar;93(3):1193–1194. doi: 10.1128/jb.93.3.1193-1194.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANDELSTAM J., ROGERS H. J. The incorporation of amino acids into the cell-wall mucopeptide of staphylococci and the effect of antibiotics on the process. Biochem J. 1959 Aug;72:654–662. doi: 10.1042/bj0720654. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MITCHELL P., MOYLE J. Autolytic release and osmotic properties of protoplasts from Staphylococcus aureus. J Gen Microbiol. 1957 Feb;16(1):184–194. doi: 10.1099/00221287-16-1-184. [DOI] [PubMed] [Google Scholar]
- NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
- NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PERKINS H. R. Chemical structure and biosynthesis of bacterial cell walls. Bacteriol Rev. 1963 Mar;27:18–55. doi: 10.1128/br.27.1.18-55.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schindler C. A. The role of NaC1 in the lysis of Staphylococcus aureus by lysostaphin. J Gen Microbiol. 1965 Aug;40(2):199–205. doi: 10.1099/00221287-40-2-199. [DOI] [PubMed] [Google Scholar]
- Virgilio R., González C., Muñoz N., Mendoza S. Electron microscopy of Staphylococcus aureus cell wall lysis. J Bacteriol. 1966 May;91(5):2018–2024. doi: 10.1128/jb.91.5.2018-2024.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIDEL W., PELZER H. BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS. Adv Enzymol Relat Areas Mol Biol. 1964;26:193–232. doi: 10.1002/9780470122716.ch5. [DOI] [PubMed] [Google Scholar]