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. 1967 Oct;94(4):821–825. doi: 10.1128/jb.94.4.821-825.1967

Nephelometric Assay of Bovine Antistaphylocoagulase Serum1

F J Stutzenberger a, C L San Clemente a
PMCID: PMC276738  PMID: 4167589

Abstract

A nephelometric assay of staphylococcal coagulase has been utilized to measure coagulase inhibition by bovine anticoagulase serum. Suitably diluted antisera produced maximal inhibition when incubated with purified coagulase at pH 7.3 in phosphate-buffered saline for 15 min at 22 C or 1 hr at 4 C. Neutralization of coagulase activity was measured as the reduction in the clotting rate of a fibrinogen-plasma substrate, and was directly proportional to the concentration of antiserum over a wide range of coagulase activity. A unit of anticoagulase was defined as the amount of inhibitor that neutralized one unit of coagulase. In addition to the heat-stable (56 C, 30 min) antibody contained in the crude γ-globulin fraction, a heat-labile, nondialyzable coagulase inhibitor was also detected in the sera from 15 of 16 randomly selected dairy cows.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLAIR J. E., CARR M. The techniques and interpretation of phage typing of staphylococci. J Lab Clin Med. 1960 Apr;55:650–662. [PubMed] [Google Scholar]
  2. BOAKE W. C. Antistaphylocoagulase in experimental staphylococcal infections. J Immunol. 1956 Feb;76(2):89–96. [PubMed] [Google Scholar]
  3. BUSSARD A. E. Reaction kinetics of the four-component system: glucose-6--phosphate dehydrogenase, substrate, coenzyme and antibody-behaviour of serum from tolerant rabbits. Ann N Y Acad Sci. 1963 May 8;103:890–901. doi: 10.1111/j.1749-6632.1963.tb53742.x. [DOI] [PubMed] [Google Scholar]
  4. CINADER B. Antibody to enzymes--a three-component system. Introduction: immunochemistry of enzymes. Ann N Y Acad Sci. 1963 May 8;103:495–548. doi: 10.1111/j.1749-6632.1963.tb53717.x. [DOI] [PubMed] [Google Scholar]
  5. CINADER B., LAFFERTY K. J. Antibody as inhibitor of ribonuclease: the role of steric hindrance, aggregate formation, and specificity. Ann N Y Acad Sci. 1963 May 8;103:653–673. doi: 10.1111/j.1749-6632.1963.tb53725.x. [DOI] [PubMed] [Google Scholar]
  6. DUTHIE E. S., LORENZ L. L. Staphylococcal coagulase; mode of action and antigenicity. J Gen Microbiol. 1952 Feb;6(1-2):95–107. doi: 10.1099/00221287-6-1-2-95. [DOI] [PubMed] [Google Scholar]
  7. FISHER M. W., DEVLIN H. B., ERLANDSON A. L. A NEW STAPHYLOCOCCAL ANTIGEN. ITS PREPARATION AND IMMUNIZING ACTIVITY AGAINST EXPERIMENTAL INFECTIONS. Nature. 1963 Sep 14;199:1074–1075. doi: 10.1038/1991074a0. [DOI] [PubMed] [Google Scholar]
  8. LOMINSKI I., SMITH D. D., SCOTT A. C., ARBUTHNOTT J. P., GRAY S., MUIR D., TURNER G. H., HEDGES C. K. Immunisation against experimental staphylococcal infection with coagulase-rich preparations. Lancet. 1962 Jun 23;1(7243):1315–1318. doi: 10.1016/s0140-6736(62)92420-0. [DOI] [PubMed] [Google Scholar]
  9. MARUCCI A. A., MAYER M. M. Quantitative studies on the inhibition of crystalline urease by rabbit anti-urease. Arch Biochem Biophys. 1955 Feb;54(2):330–340. doi: 10.1016/0003-9861(55)90044-8. [DOI] [PubMed] [Google Scholar]
  10. MAURER P. H. Nature of antigenic determinants in proteins and synthetic polypeptides. Ann N Y Acad Sci. 1963 May 8;103:549–581. doi: 10.1111/j.1749-6632.1963.tb53718.x. [DOI] [PubMed] [Google Scholar]
  11. Roberts D. B. Immunochemical and enzymatic studies and glutamate dehydrogenase and a related mutant protein from Neurospora crassa. J Bacteriol. 1966 May;91(5):1888–1895. doi: 10.1128/jb.91.5.1888-1895.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SAMUELS A. Immunoenzymology-reaction processes, kinetics and the role of conformational alteration. Ann N Y Acad Sci. 1963 May 8;103:858–889. doi: 10.1111/j.1749-6632.1963.tb53741.x. [DOI] [PubMed] [Google Scholar]
  13. Stutzenberger F. J., Clemente C. L., Vadehra D. V. Nephelometric assay of staphylococal coagulase. J Bacteriol. 1966 Oct;92(4):1005–1009. doi: 10.1128/jb.92.4.1005-1009.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TSUJI F. I., GINDLER E. M., DAVIS D. L. Luciferase-antiluciferase. Ann N Y Acad Sci. 1963 May 8;103:715–723. doi: 10.1111/j.1749-6632.1963.tb53728.x. [DOI] [PubMed] [Google Scholar]
  15. Tager M., Drummond M. C. Staphylocoagulase. Ann N Y Acad Sci. 1965 Jul 23;128(1):92–111. doi: 10.1111/j.1749-6632.1965.tb11632.x. [DOI] [PubMed] [Google Scholar]
  16. ZOLLI Z., Jr, SANCLEMENTE C. L. PURIFICATION AND CHARACTERIZATION OF STAPHYLOCOAGULASE. J Bacteriol. 1963 Sep;86:527–535. doi: 10.1128/jb.86.3.527-535.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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