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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Virology. 2009 Sep 8;394(1):82–90. doi: 10.1016/j.virol.2009.08.012

Figure 2. LAP blocks HCV 5′ UTR-protein interaction of a number of polypeptides from Huh-7.5 cells .

Figure 2

(A) An S10 cell-free translation lysate from Huh-7.5 cells was used for RNA-protein interaction by UV-crosslinking assay using radiolabeled HCV 5′UTR RNA. UV-crosslinked protein-nucleotidyl complexes in the absence (lane1) and presence (lanes 2–6) of S10 containing 40 and 60 μM LAP (lanes 3 and 4) or a non-specific peptide, NSP (lanes 5 and 6) were analyzes by SDS-PAGE. (B) Three pooled reactions as in lane 2 above were used for individual immunoprecipitation of protein-nucleotidyl complexes with anti-La (lane 1), anti-PTB (lane 2), and anti-PCBP2 (lane 3) antibodies.