Abstract
A permeaseless mutant of Escherichia coli, which produces β-galactosidase constitutively, was treated briefly with ethylenediaminetetraacetate and then with the phospholipases of Bacillus cereus. Cell lysis occurred, as indicated by an increase in β-galactosidase activity and a decrease in absorbancy of the cell suspension. The susceptibility of the cells to attack by ethylenediaminetetraacetate and the phospholipases was markedly affected by the age of the cells when harvested. The results suggest that permeability changes may be associated with the activity of a phospholipase that specifically degrades phosphatidyl ethanolamine. A sonic-treatment method for determining the total β-galactosidase content of E. coli cells, which is independent of their age when harvested, is described.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANGHAM A. D., DAWSON R. M. Electrokinetic requirements for the reaction between Cl. perfringens alpha-toxin (phospholipase C) and phospholipid substrates. Biochim Biophys Acta. 1962 May 7;59:103–115. doi: 10.1016/0006-3002(62)90701-1. [DOI] [PubMed] [Google Scholar]
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- BROCK T. D., BROCK M. L. Effect of novobiocin on permeability of Escherichia coli. Arch Biochem Biophys. 1959 Nov;85:176–185. doi: 10.1016/0003-9861(59)90461-8. [DOI] [PubMed] [Google Scholar]
- COHN M. Contributions of studies on the beta-galactosidase of Escherichia coli to our understanding of enzyme synthesis. Bacteriol Rev. 1957 Sep;21(3):140–168. doi: 10.1128/br.21.3.140-168.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fowler A. V., Zabin I. Effects of Dimethylsulfoxide on the Lactose Operon in Escherichia coli. J Bacteriol. 1966 Aug;92(2):353–357. doi: 10.1128/jb.92.2.353-357.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HORIUCHI T., TOMIZAWA J. I., NOVICK A. Isolation and properties of bacteria capable of high rates of beta-galactosidase synthesis. Biochim Biophys Acta. 1962 Jan 22;55:152–163. doi: 10.1016/0006-3002(62)90941-1. [DOI] [PubMed] [Google Scholar]
- Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
- LEIVE L. A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA. Proc Natl Acad Sci U S A. 1965 Apr;53:745–750. doi: 10.1073/pnas.53.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leive L. Release of lipopolysaccharide by EDTA treatment of E. coli. Biochem Biophys Res Commun. 1965 Nov 22;21(4):290–296. doi: 10.1016/0006-291x(65)90191-9. [DOI] [PubMed] [Google Scholar]
- PAIGEN K. CHANGES IN THE INDUCIBILITY OF GALACTOKINASE AND BETA-GALACTOSIDASE DURING INHIBITION OF GROWTH IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Oct 1;77:318–328. doi: 10.1016/0006-3002(63)90502-x. [DOI] [PubMed] [Google Scholar]
- Reithel F. J., Newton R. M., Eagleson M. Effects of thiols on escherichia coli beta-galactosidases. Nature. 1966 Jun 18;210(5042):1265–1265. doi: 10.1038/2101265a0. [DOI] [PubMed] [Google Scholar]
- SLEIN M. W., LOGAN G. F., Jr Partial purification and properties of two phospholipases of Bacillus cereus. J Bacteriol. 1963 Feb;85:369–381. doi: 10.1128/jb.85.2.369-381.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slein M. W., Logan G. F. Characterization of the Phospholipases of Bacillus cereus and Their Effects on Erythrocytes, Bone, and Kidney Cells. J Bacteriol. 1965 Jul;90(1):69–81. doi: 10.1128/jb.90.1.69-81.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]