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. 1967 Oct;94(4):1088–1092. doi: 10.1128/jb.94.4.1088-1092.1967

Effects of Mitomycin C and Thymidine Deprivation on Lysogenic and Sensitive Strains of Bacillus megaterium1

W F Maisch a,2, J T Wachsman a,3
PMCID: PMC276780  PMID: 4963773

Abstract

A triple auxotroph of Bacillus megaterium strain KM was lysogenized with a phage suspension from B. megaterium 899a. The lysogenic and phage-sensitive derivatives of KM were found to die at the same exponential rate during thymineless incubation, despite the fact that the lysogenic strain became induced. The lysogenic strain was also induced by mitomycin C, and died at an exponential rate which was approximately twice that of the sensitive strain. With both strains, the lethality of mitomycin C was the same in the presence and absence of thymidine; thymidine was required for maximal phage production. Mitomycin C preferentially inhibited deoxyribonucleic acid (DNA) synthesis of both strains for the first 60 min. The (DNA) synthetic ability of the lysogenic strain was subsequently restored, due to phage production. Since there was no evidence that sensitive strains of KM contained other inducible elements (prophage or probacteriocins), it is concluded that both thymineless death and mitomycin C death can occur via mechanisms not involving induction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ENDO H., AYABE K., AMAKO K., TAKEYA K. INDUCIBLE PHAGE OF ESCHERICHIA COLI 15. Virology. 1965 Mar;25:469–471. doi: 10.1016/0042-6822(65)90067-x. [DOI] [PubMed] [Google Scholar]
  2. FRAMPTON E. W., BRINKLEY B. R. EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:446–452. doi: 10.1128/jb.90.2.446-452.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ishibashi M., Hirota Y. Hybridization between Escherichia coli K-12 and 15T- and thymineless death of their derivatives. J Bacteriol. 1965 Nov;90(5):1496–1497. doi: 10.1128/jb.90.5.1496-1497.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KORN D., WEISSBACH A. Thymineless induction in Escherichia coli K12 (lambda). Biochim Biophys Acta. 1962 Nov 26;61:775–790. doi: 10.1016/0926-6550(62)90060-9. [DOI] [PubMed] [Google Scholar]
  5. LEVINE M. Effect of mitomycin C on interactions between temperate phages and bacteria. Virology. 1961 Apr;13:493–499. doi: 10.1016/0042-6822(61)90280-x. [DOI] [PubMed] [Google Scholar]
  6. MAISCH W. F., WACHSMAN J. T. THYMINELESS INDUCTION OF BACTERIOPHAGE IN BACILLUS MEGATERIUM. J Bacteriol. 1964 Nov;88:1388–1393. doi: 10.1128/jb.88.5.1388-1393.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MANGALO R., WACHSMAN J. T. Effect of 8-azaguanine on growth and viability of Bacillus megaterium. J Bacteriol. 1962 Jan;83:27–34. doi: 10.1128/jb.83.1.27-34.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MARJAI E., IVANOVICS G. THE EFFECT OF DIFFERENT ANTICANCER AGENTS ON INDUCIBLE SYSTEMS OF BACILLUS MEGATERIUM. Acta Microbiol Acad Sci Hung. 1964;11:193–198. [PubMed] [Google Scholar]
  9. MELECHEN N. E., SKAAR P. D. The provocation of an early step of induction by thymine deprivation. Virology. 1962 Jan;16:21–29. doi: 10.1016/0042-6822(62)90198-8. [DOI] [PubMed] [Google Scholar]
  10. Mennigmann H. D. Electron microscopy of the anti-bacterial agent produced by Escherichia coli 15. J Gen Microbiol. 1965 Nov;41(2):151–154. doi: 10.1099/00221287-41-2-151. [DOI] [PubMed] [Google Scholar]
  11. Mennigmann H. D. Induction in Escherichia coli 15 of the colicinogenic factor by thymine-less death. Biochem Biophys Res Commun. 1964 Jul 1;16(4):373–378. doi: 10.1016/0006-291x(64)90043-9. [DOI] [PubMed] [Google Scholar]
  12. Muschel L. H., Schmoker K. Activity of mitomycin C, other antibiotics, and serum against lysogenic bacteria. J Bacteriol. 1966 Oct;92(4):967–971. doi: 10.1128/jb.92.4.967-971.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OTSUJI N., SEKIGUCHI M., IIJIMA T., TAKAGI Y. Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Nature. 1959 Oct 3;184(Suppl 14):1079–1080. doi: 10.1038/1841079b0. [DOI] [PubMed] [Google Scholar]
  14. Rolfe R. On the mechanism of thymineless death in Bacillus subtilis. Proc Natl Acad Sci U S A. 1967 Jan;57(1):114–121. doi: 10.1073/pnas.57.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SEAMAN E., TARMY E., MARMUR J. INDUCIBLE PHAGES OF BACILLUS SUBTILIS. Biochemistry. 1964 May;3:607–613. doi: 10.1021/bi00893a001. [DOI] [PubMed] [Google Scholar]
  16. SICARD N., DEVORET R. [Effects of thymine dificiency on the K 12 T lysogenic and 15 T colicinogenic strains of Escherichia coli]. C R Hebd Seances Acad Sci. 1962 Sep 10;255:1417–1419. [PubMed] [Google Scholar]
  17. SICARD N. IMPORTANCE DE L'INDUCTION PAR CARENCE EN THYMINE SUR LA VIABILIT'E DES BACT'ERIES EXIGEANTES EN THYMINE. C R Hebd Seances Acad Sci. 1964 Sep 21;259:2040–2042. [PubMed] [Google Scholar]
  18. Wachsman J. T., Hogg L. Use of thymineless death to enrich for doubly auxotrophic mutants of Bacillus megaterium. J Bacteriol. 1964 May;87(5):1118–1122. doi: 10.1128/jb.87.5.1118-1122.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wachsman J. T., Kemp S., Hogg L. Comparative effects of 5-fluorouracil on strains of Bacillus megaterium. J Bacteriol. 1964 May;87(5):1011–1018. doi: 10.1128/jb.87.5.1011-1018.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wachsman J. T., Kemp S., Kogg L. Thymineless death in Bacillus megaterium. J Bacteriol. 1964 May;87(5):1079–1086. doi: 10.1128/jb.87.5.1079-1086.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

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