Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Oct;94(4):1236–1243. doi: 10.1128/jb.94.4.1236-1243.1967

Fine-Structure Analysis of Intercellular and Intracellular Mitochondrial Diversity in Saccharomyces cerevisiae

Micheline Federman 1, Charlotte J Avers 1
PMCID: PMC276798  PMID: 4293081

Abstract

Crosses were made between haploid wild-type and suppressive petite strains of bakers' yeast to obtain zygotes for analysis of mitochondrial heterogeneity. Wild-type × petite zygotes contained about 40% noncristate mitochondria when immediate mating mixtures were examined. The frequency of defective mitochondria had decreased to an average of 9.2% in 1-week-old zygote isolate cultures, and to 4.4% in slant cultures 1.5 years after initial zygote isolation. The latter value was not significantly different from values obtained with wild × wild zygotes of either age. The noncristate mitochondria were of two types: one lacking inner membrane invaginations or elaborations and the other containing concentrically arranged loops of inner membrane. The significance of these two types of respiration-deficient mitochondria is unknown. The gradual decrease in frequency of noncristate mitochondria, perhaps due to selection pressures in mixed chondriomes, was discussed as a further indication of the semiautonomous nature of the yeast organelle.

Full text

PDF
1236

Images in this article

1238Image
on p.1238
1239Image
on p.1239
1239Image
on p.1239
1241Image
on p.1241
1241Image
on p.1241

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVERS C. J., LIN F. H., PFEFFER C. R. HISTOCHEMICAL STUDIES OF MITOCHONDRIAL VARIATION DURING AEROBIC GROWTH OF RESPIRATION-NORMAL BAKER'S YEAST. J Histochem Cytochem. 1965 May-Jun;13:344–349. doi: 10.1177/13.5.344. [DOI] [PubMed] [Google Scholar]
  2. AVERS C. J., PFEFFER C. R., RANCOURT M. W. ACRIFLAVINE INDUCTION OF DIFFERENT KINDS OF "PETITE" MITOCHONDRIAL POPULATIONS IN SACCHAROMYCES CEREVISIAE. J Bacteriol. 1965 Aug;90:481–494. doi: 10.1128/jb.90.2.481-494.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avers C. J., Rancourt M. W., Lin F. H. Intracellular mitochondrial diversity in various strains of Saccgarintces cerevisiae. Proc Natl Acad Sci U S A. 1965 Aug;54(2):527–535. doi: 10.1073/pnas.54.2.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BATES H. M., SIMPSON M. V. The net synthesis of cytochrome c in calf-heart mitochondria. Biochim Biophys Acta. 1959 Apr;32:597–599. doi: 10.1016/0006-3002(59)90658-4. [DOI] [PubMed] [Google Scholar]
  5. Barnett W. E., Brown D. H. Mitochondrial transfer ribonucleic acids. Proc Natl Acad Sci U S A. 1967 Feb;57(2):452–458. doi: 10.1073/pnas.57.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brock T. D. Biochemical and cellular changes occuring during conjugation in Hansenula wingei. J Bacteriol. 1965 Oct;90(4):1019–1025. doi: 10.1128/jb.90.4.1019-1025.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CONTI S. F., BROCK T. D. ELECTRON MICROSCOPY OF CELL FUSION IN CONJUGATING HANSENULA WINGEI. J Bacteriol. 1965 Aug;90:524–533. doi: 10.1128/jb.90.2.524-533.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EPHRUSS B., GRANDCHAMP S. ETUDES SUR LA SUPPRESSIVIT'E DES MUTANTS 'A DEFICIENCE RESPIRATOIRE DE LA LEVURE. I. EXISTENCE AU NIVEAU CELLULAIRE DE DIVERS "DEGR'ES DE SUPPRESSIVIT'E". Heredity (Edinb) 1965 Feb;20:1–7. doi: 10.1038/hdy.1965.1. [DOI] [PubMed] [Google Scholar]
  9. EPHRUSSI B., HOTTINGUER H. On an unstable cell state in yeast. Cold Spring Harb Symp Quant Biol. 1951;16:75–85. doi: 10.1101/sqb.1951.016.01.007. [DOI] [PubMed] [Google Scholar]
  10. Ephrussi B., Jakob H., Grandchamp S. Etudes Sur La SuppressivitE Des Mutants a Deficience Respiratoire De La Levure. II. Etapes De La Mutation Grande En Petite Provoquee Par Le Facteur Suppressif. Genetics. 1966 Jul;54(1):1–29. doi: 10.1093/genetics/54.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ephrussi B., de Margerie-Hottinguer H., Roman H. SUPPRESSIVENESS: A NEW FACTOR IN THE GENETIC DETERMINISM OF THE SYNTHESIS OF RESPIRATORY ENZYMES IN YEAST. Proc Natl Acad Sci U S A. 1955 Dec 15;41(12):1065–1071. doi: 10.1073/pnas.41.12.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GIBOR A., GRANICK S. PLASTIDS AND MITOCHONDRIA: INHERITABLE SYSTEMS. Science. 1964 Aug 14;145(3633):890–897. doi: 10.1126/science.145.3635.890. [DOI] [PubMed] [Google Scholar]
  13. LUCK D. J., REICH E. DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1964 Oct;52:931–938. doi: 10.1073/pnas.52.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. doi: 10.1083/jcb.11.3.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Matile P., Wiemken A. The vacuole as the lysosome of the yeast cell. Arch Mikrobiol. 1967 Feb 20;56(2):148–155. doi: 10.1007/BF00408765. [DOI] [PubMed] [Google Scholar]
  17. Mounolou J. C., Jakob H., Slonimski P. P. Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations. Biochem Biophys Res Commun. 1966 Jul 20;24(2):218–224. doi: 10.1016/0006-291x(66)90723-6. [DOI] [PubMed] [Google Scholar]
  18. NAGAI S. Induction of the respiration-deficient mutation in yeast by various synthetic dyes. Science. 1959 Oct 30;130(3383):1188–1189. doi: 10.1126/science.130.3383.1188-a. [DOI] [PubMed] [Google Scholar]
  19. Reich E., Luck D. J. Replication and inheritance of mitochondrial DNA. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1600–1608. doi: 10.1073/pnas.55.6.1600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SHERMAN F., EPHRUSSI B. The relationship between respiratory deficiency and suppressiveness in yeast as determined with segregational mutants. Genetics. 1962 Jun;47:695–700. doi: 10.1093/genetics/47.6.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
  22. SOMMER J. R., BLUM J. J. CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASES IN EUGLENA GRACILIS. J Cell Biol. 1965 Feb;24:235–251. doi: 10.1083/jcb.24.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stone G. E., Miller O. L., Jr A stable mitochondrial DNA in Tetrahymena pyriformis. J Exp Zool. 1965 Jun;159(1):33–37. doi: 10.1002/jez.1401590104. [DOI] [PubMed] [Google Scholar]
  24. Wintersberger E. Occurrence of a DNA-polymerase in isolated yeast mitochondria. Biochem Biophys Res Commun. 1966 Oct 5;25(1):1–7. doi: 10.1016/0006-291x(66)90630-9. [DOI] [PubMed] [Google Scholar]
  25. Woodward D. O., Munkres K. D. Alterations of a maternally inherited mitochondrial structural protein in respiratory-deficient strains of Neurospora. Proc Natl Acad Sci U S A. 1966 Apr;55(4):872–880. doi: 10.1073/pnas.55.4.872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. YOTSUYANAGI Y. [Study of yeast mitochondria. I. Variations in mitochondrial ultrastructure during the aerobic growth cycle]. J Ultrastruct Res. 1962 Aug;7:121–140. doi: 10.1016/s0022-5320(62)80031-8. [DOI] [PubMed] [Google Scholar]
  27. YOTSUYANAGI Y. [Study of yeast mitochondria. II. Mitochondria of respiration-deficient mutants]. J Ultrastruct Res. 1962 Aug;7:141–158. doi: 10.1016/s0022-5320(62)80032-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES