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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Virology. 2008 Dec 10;385(1):183–191. doi: 10.1016/j.virol.2008.11.018

Fig. 4. Lyn degradation by c-Cbl RING.

Fig. 4

(A) CHO-K1 cells were transfected with 1 μg of Lyn and indicated amount of c-Cbl-Flag. Cell lysates were immunoblotted for Lyn and c-Cbl. (B) CHO-K1 cells were transfected with Lyn, wild type or mutant c-Cbl-Flag, and Ub-His. Lyn immunoprecipitates were immunoblotted with anti-His antibody to detect ubiquitin-conjugated Lyn. (C) Transfected CHO-K1 cells were treated with 10 μM MG132 and immunoblotted for Lyn and c-Cbl. The data is the representative of three comparable experiments.