Abstract
By a simple modification, common commercial dehydrated laboratory media can be used for the evaluation of fluorescent pigment excretion by Pseudomonas species. The addition of sufficient sterile egg white or of the iron-binding egg white protein, conalbumin, to bind all the iron in these media converted them to efficient diagnostic media. The amount of egg white found to be satisfactory was 10% of the final medium volume. Its conalbumin equivalent was 1.7 mg of conalbumin per ml of final medium. Such modified media are at least the equivalent of the medium recommended for the evaluation of this important characteristic.
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- GARIBALDI J. A., NEILANDS J. B. Formation of iron-binding compounds by micro-organisms. Nature. 1956 Mar 17;177(4507):526–527. doi: 10.1038/177526a0. [DOI] [PubMed] [Google Scholar]
- Georgia F. R., Poe C. F. Study of Bacterial Fluorescence in Various Media: I. Inorganic Substances Necessary for Bacterial Fluorescence. J Bacteriol. 1931 Nov;22(5):349–361. doi: 10.1128/jb.22.5.349-361.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Georgia F. R., Poe C. F. Study of Bacterial Fluorescence in Various Media: II. The Production of Fluorescence in Media Made from Peptones. J Bacteriol. 1932 Feb;23(2):135–145. doi: 10.1128/jb.23.2.135-145.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
- LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PATON A. M. Enhancement of pigment production by Pseudomonas. Nature. 1959 Oct 17;184(Suppl 16):1254–1254. doi: 10.1038/1841254a0. [DOI] [PubMed] [Google Scholar]
- Schade A. L., Caroline L. RAW HEN EGG WHITE AND THE ROLE OF IRON IN GROWTH INHIBITION OF SHIGELLA DYSENTERIAE, STAPHYLOCOCCUS AUREUS, ESCHERICHIA COLI AND SACCHAROMYCES CEREVISIAE. Science. 1944 Jul 7;100(2584):14–15. doi: 10.1126/science.100.2584.14. [DOI] [PubMed] [Google Scholar]
- TOTTER J. R., MOSELEY F. T. Influence of the concentration of iron on the production of fluorescin by Pseudomonas aeruginosa. J Bacteriol. 1953 Jan;65(1):45–47. doi: 10.1128/jb.65.1.45-47.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]