Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Nov;94(5):1400–1405. doi: 10.1128/jb.94.5.1400-1405.1967

Serological Comparison of Spherules and Arthrospores of Coccidioides immitis1

Marshall E Landay 1,2,2, Robert W Wheat 1,2, Norman F Conant 1,2, Edwin P Lowe 1,2
PMCID: PMC276838  PMID: 6057798

Abstract

Spherule and arthrospore cellular preparations were sonic-treated and separated into their respective supernatant and sediment components. Complement-fixation tests with antispherule and antiarthrospore pooled rabbit sera revealed that the soluble antigens exhibited more serological activity than the sediment preparations. After autoclaving, an arthrospore cellular antigen exhibited increased activity with either antisera, whereas autoclaved spherules exhibited increased activity only with antispherule serum. Complement-fixation tests with coccicioidin and spherule culture supernatant preparations revealed quantitative or qualitative differences in antigenic determinants between these two morphological phases of Coccidioides immitis.

Full text

PDF
1400

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AJELLO L., WALLS K., MOORE J. C., FALCONE R. Rapid production of complement fixation antigens for systemic mycotic diseases. I. Coccidioidin: influence of media and mechanical agitation on its development. J Bacteriol. 1959 Jun;77(6):753–756. doi: 10.1128/jb.77.6.753-756.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROSBE E. A., KIETZMAN J. N., KURNICK N. B. COMPLEMENT-FIXATION TITERS IN EXPERIMENTAL COCCIDIOIDOMYCOSIS IN RABBITS. J Bacteriol. 1964 Jul;88:233–241. doi: 10.1128/jb.88.1.233-241.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CAMPBELL C. C. The accuracy of serologic methods in diagnosis. Ann N Y Acad Sci. 1960 Aug 27;89:163–177. doi: 10.1111/j.1749-6632.1960.tb20139.x. [DOI] [PubMed] [Google Scholar]
  4. CONVERSE J. L. Effect of surface active agents on endosporulation of Coccidioides immitis in a chemically defined medium. J Bacteriol. 1957 Jul;74(1):106–107. doi: 10.1128/jb.74.1.106-107.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HILL G. B., CAMPBELL C. C. A further evaluation of histoplasmin and yeast phase antigen of Histoplasma capsulatum in the complement fixation test. J Lab Clin Med. 1956 Aug;48(2):255–263. [PubMed] [Google Scholar]
  6. KONG Y. C., LEVINE H. B., SMITH C. E. Immunogenic properties of nondisrupted and disrupted spherules of Coccidioides immitis in mice. Sabouraudia. 1963 Feb;2:131–142. [PubMed] [Google Scholar]
  7. Kong Y. C., Levine H. B. Experimentally induced immunity in the mycoses. Bacteriol Rev. 1967 Mar;31(1):35–53. doi: 10.1128/br.31.1.35-53.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEVINE H. B., COBB J. M., SMITH C. E. Immunity to coccidioi-domycosis induced in mice by purified spherule, arthrospore, and mycelial vaccines. Trans N Y Acad Sci. 1960 Apr;22:436–449. doi: 10.1111/j.2164-0947.1960.tb00711.x. [DOI] [PubMed] [Google Scholar]
  9. LEVINE H. B. Purification of the spherule-endospore phase of Coccidioides immitis. Sabouraudia. 1961 Jun;1:112–115. doi: 10.1080/00362176285190231. [DOI] [PubMed] [Google Scholar]
  10. Landay M. E., Wheat R. W., Conant N. F., Lowe E. P. Serological comparison of the three morphological phases of Coccidioides immitis by the agar gel diffusion method. J Bacteriol. 1967 Jan;93(1):1–6. doi: 10.1128/jb.93.1.1-6.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MARTIN D. S. Serologic studies on North American blastomycosis; studies with soluble antigens from untreated and sonic-treated yeast-phase cells of Blastomyces dermatitidis. J Immunol. 1953 Oct;71(4):192–201. [PubMed] [Google Scholar]
  12. PAPPAGIANIS D., PUTMAN E. W., KOBAYASHI G. S. Polysaccharide of Coccidioides immitis. J Bacteriol. 1961 Nov;82:714–723. doi: 10.1128/jb.82.5.714-723.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PINE L., PEACOCK C. L. Studies on the growth of Histoplasma capsulatum. IV. Factors influencing conversion of the mycelial phase to the yeast phase. J Bacteriol. 1958 Feb;75(2):167–174. doi: 10.1128/jb.75.2.167-174.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SINSKI J. T., LOWE E. P., CONANT N. F., HARDIN H. F., CASTLEBERRY M. W., RAY J. G., Jr IMMUNIZATION AGAINST EXPERIMENTAL LETHAL SIMIAN COCCIDIOIDOMYCOSIS USING WHOLE KILLED ARTHROSPORES AND CELL FRACTION. Mycologia. 1965 May-Jun;57:431–441. [PubMed] [Google Scholar]
  15. SMITH C. E., SAITO M. T., BEARD R. R., KEPP R. M., CLARK R. W., EDDIE B. U. Serological tests in the diagnosis and prognosis of coccidioidomycosis. Am J Hyg. 1950 Jul;52(1):1–21. doi: 10.1093/oxfordjournals.aje.a119404. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES