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. 1967 Nov;94(5):1411–1416. doi: 10.1128/jb.94.5.1411-1416.1967

Physicochemical and Biological Properties of Sonically Treated Vi Antigen

Donald G Martin 1,2,1, Francis G Jarvis 1,2, Kelsey C Milner 1,2
PMCID: PMC276840  PMID: 4964477

Abstract

Electrophoretically purified Vi antigen from Citrobacter freundii 5396/38 was depolymerized by sonic treatment. The treatment caused an 80% reduction in specific viscosity and a reduction in molecular weight from 1.6 × 106 to 3.9 × 104. The O-acetyl and N-acetyl contents of the antigen and its infrared spectrum remained unchanged. The sonically treated antigen was only 1% as effective as the original antigen in eliciting protection in mice against challenge with Salmonella typhi. Sonically treated antigen also elicited lower antibody titers after single injections in mice and rabbits. No loss in ability to precipitate antibody or to sensitize red blood cells for hemagglutination was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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