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. 1967 Nov;94(5):1425–1430. doi: 10.1128/jb.94.5.1425-1430.1967

Role of the Phagocyte in Host-Parasite Interactions XII. Hydrogen Peroxide-Myeloperoxidase Bactericidal System in the Phagocyte

R J McRipley 1, A J Sbarra 2
PMCID: PMC276842  PMID: 4964478

Abstract

An antimicrobial system in polymorphonuclear neutrophils (PMN) consisting of myeloperoxidase and hydrogen peroxide has been proposed. This system appears to be activated during phagocytosis as a result of the stimulated metabolic activities. A lysed-granules (LG) fraction was prepared from guinea pig exudative PMN. LG alone possessed bactericidal activity which was related to the pH of the reaction; the lower the pH, the more marked the activity. When low concentrations of both H2O2 and LG were combined under conditions where neither factor alone exhibited significant killing power, there was a striking increase in bactericidal activity. This enhanced activity was much greater than an additive effect. The LG-peroxide antibacterial system was most active over a pH range of 4.0 to 6.0. The activity of the LG-peroxide system was essentially abolished by peroxidase inhibitors, NaN3, KCN, and aminotriazole. The antibacterial activity of this system was nonspecific in nature, being equally effective against gram-negative and gram-positive organisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHN Z. A., HIRSCH J. G. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J Exp Med. 1960 Dec 1;112:983–1004. doi: 10.1084/jem.112.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cline M. J. Metabolism of the circulating leukocyte. Physiol Rev. 1965 Oct;45(4):674–720. doi: 10.1152/physrev.1965.45.4.674. [DOI] [PubMed] [Google Scholar]
  3. HIRSCH J. G., COHN Z. A. Degranulation of polymorphonuclear leucocytes following phagocytosis of microorganisms. J Exp Med. 1960 Dec 1;112:1005–1014. doi: 10.1084/jem.112.6.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HIRSCH J. G. Phagocytin: a bactericidal substance from polymorphonuclear leucocytes. J Exp Med. 1956 May 1;103(5):589–611. doi: 10.1084/jem.103.5.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HIRSCH J. G. Studies of the bactericidal action of phagocytin. J Exp Med. 1956 May 1;103(5):613–621. doi: 10.1084/jem.103.5.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirsch J. G. Phagocytosis. Annu Rev Microbiol. 1965;19:339–350. doi: 10.1146/annurev.mi.19.100165.002011. [DOI] [PubMed] [Google Scholar]
  7. Klebanoff S. J., Clem W. H., Luebke R. G. The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system. Biochim Biophys Acta. 1966 Mar 28;117(1):63–72. doi: 10.1016/0304-4165(66)90152-8. [DOI] [PubMed] [Google Scholar]
  8. MAEHLY A. C., CHANCE B. The assay of catalases and peroxidases. Methods Biochem Anal. 1954;1:357–424. doi: 10.1002/9780470110171.ch14. [DOI] [PubMed] [Google Scholar]
  9. McRipley R. J., Sbarra A. J. Role of the phagocyte in host-parasite interactions. XI. Relationship between stimulated oxidative metabolism and hydrogen peroxide formation, and intracellular killing. J Bacteriol. 1967 Nov;94(5):1417–1424. doi: 10.1128/jb.94.5.1417-1424.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rowley D. Phagocytosis and immunity. The carrier state and cellular immunity. Experientia. 1966 Jan 15;22(1):9–13. [PubMed] [Google Scholar]
  11. SBARRA A. J., BARDAWIL W. A., SHIRLEY W., GILFILLAN R. F. Degranulation of guinea pig leukocytes accompanying phagocytosis. Exp Cell Res. 1961 Sep;24:609–611. doi: 10.1016/0014-4827(61)90466-9. [DOI] [PubMed] [Google Scholar]
  12. Schultz J., Corlin R., Oddi F., Kaminker K., Jones W. Myeloperoxidase of the leucocyte of normal human blood. 3. Isolation of the peroxidase granule. Arch Biochem Biophys. 1965 Jul;111(1):73–79. doi: 10.1016/0003-9861(65)90324-3. [DOI] [PubMed] [Google Scholar]
  13. Stefanovic J., Absolonová O. Antibacterial and proteolytic activity of rabbit phagocytes and the effect of temperature and endotoxin on this activity. Folia Microbiol (Praha) 1966;11(5):358–363. doi: 10.1007/BF02887692. [DOI] [PubMed] [Google Scholar]
  14. Weissmann G. Lysosome. N Engl J Med. 1965 Nov 11;273(20):1084–contd. doi: 10.1056/NEJM196511112732006. [DOI] [PubMed] [Google Scholar]
  15. Zeya H. I., Spitznagel J. K. Cationic proteins of polymorphonuclear leukocyte lysosomes. I. Resolution of antibacterial and enzymatic activities. J Bacteriol. 1966 Feb;91(2):750–754. doi: 10.1128/jb.91.2.750-754.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zeya H. I., Spitznagel J. K. Cationic proteins of polymorphonuclear leukocyte lysosomes. II. Composition, properties, and mechanism of antibacterial action. J Bacteriol. 1966 Feb;91(2):755–762. doi: 10.1128/jb.91.2.755-762.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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