Abstract
Growing cultures of Streptococcus faecalis at temperatures above 30 C have activation energies for both rates of growth and glycolysis of 10.3 kcal mole−1, and a constant growth yield; when growth takes place below this temperature, the growth yield decreases and the activation energy for growth increases to 21.1 kcal mole−1, but the activation energy for glycolysis is unchanged. The adenosine triphosphate pool in the organisms behaves differently above and below 30 C, suggesting that the energetic coupling between anabolism and catabolism is less effective below 30 C. Washed suspensions of S. faecalis have repressed glycolytic activity and an activation energy for glycolysis of 15.6 kcal mole−1 over the whole temperature range studied. Growing cultures of Zymomonas mobilis below 33 C have a constant growth yield of 8.3 g (dry weight) of organisms per mole of glucose degraded, and activation energies for both glycolysis and growth of 11.1 kcal mole−1; above this temperature, the growth yield falls, the activation energy for growth changes to −6.9 kcal mole−1, but the activation energy for glycolysis is unchanged, so that the coupling between anabolism and catabolism is less effective above 33 C. The findings support the view that energy turnover in these bacteria is not well regulated.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUCHOP T., ELSDEN S. R. The growth of micro-organisms in relation to their energy supply. J Gen Microbiol. 1960 Dec;23:457–469. doi: 10.1099/00221287-23-3-457. [DOI] [PubMed] [Google Scholar]
- Bubela B., Holdsworth E. S. Amino acid uptake, protein and nucleic acid synthesis and turnover in Bacillus stearothermophilus. Biochim Biophys Acta. 1966 Aug 17;123(2):364–375. doi: 10.1016/0005-2787(66)90289-9. [DOI] [PubMed] [Google Scholar]
- Davey C. B., Miller R. J. Correlation of temperature-dependent water properties and the growth of bacteria. Nature. 1966 Feb 5;209(5023):638–638. doi: 10.1038/209638a0. [DOI] [PubMed] [Google Scholar]
- FORREST W. W., WALKER D. J. CHANGE IN ENTROPY DURING BACTERIAL METABOLISM. Nature. 1964 Jan 4;201:49–52. doi: 10.1038/201049a0. [DOI] [PubMed] [Google Scholar]
- FORREST W. W., WALKER D. J., STOWARD P. J. Thermodynamics of biological growth. Nature. 1962 Dec 8;196:990–992. doi: 10.1038/196990a0. [DOI] [PubMed] [Google Scholar]
- FORREST W. W., WALKER D. J. SYNTHESIS OF RESERVE MATERIALS FOR ENDOGENOUS METABOLISM IN STREPTOCOCCUS FAECALIS. J Bacteriol. 1965 Jun;89:1448–1452. doi: 10.1128/jb.89.6.1448-1452.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forrest W. W. Adenosine triphosphate pool during the growth cycle in Streptococcus faecalis. J Bacteriol. 1965 Oct;90(4):1013–1018. doi: 10.1128/jb.90.4.1013-1018.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forrest W. W., Walker D. J. Control of glycolysis in washed suspensions of Streptococcus faecalis. Nature. 1965 Jul 3;207(992):46–48. doi: 10.1038/207046a0. [DOI] [PubMed] [Google Scholar]
- GIBBS M., DEMOSS R. D. Anaerobic dissimilation of C14-labeled glucose and fructose by Pseudomonas lindneri. J Biol Chem. 1954 Apr;207(2):689–694. [PubMed] [Google Scholar]
- Pirt S. J. The maintenance energy of bacteria in growing cultures. Proc R Soc Lond B Biol Sci. 1965 Oct 12;163(991):224–231. doi: 10.1098/rspb.1965.0069. [DOI] [PubMed] [Google Scholar]
- ROSENBERGER R. F., ELSDEN S. R. The yields of Streptococcus faecalis grown in continuous culture. J Gen Microbiol. 1960 Jun;22:726–739. doi: 10.1099/00221287-22-3-726. [DOI] [PubMed] [Google Scholar]
- SENEZ J. C. Some considerations on the energetics of bacterial growth. Bacteriol Rev. 1962 Jun;26:95–107. [PMC free article] [PubMed] [Google Scholar]
- STRANGE R. E., WADE H. E., DARK F. A. EFFECT OF STARVATION ON ADENOSINE TRIPHOSPHATE CONCENTRATION IN AEROBACTER AEROGENES. Nature. 1963 Jul 6;199:55–57. doi: 10.1038/199055a0. [DOI] [PubMed] [Google Scholar]