Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Nov;94(5):1492–1496. doi: 10.1128/jb.94.5.1492-1496.1967

Genetic and Biochemical Studies on Mannose-Negative Mutants That Are Deficient in Phosphomannose Isomerase in Escherichia coli K-12

Alvin Markovitz 1, Robert J Sydiskis 1, Michael M Lieberman 1
PMCID: PMC276853  PMID: 4862193

Abstract

Two mannose-negative mutants of Escherichia coli K-12 have been isolated. These mutants are deficient in the ability to synthesize phosphomannose isomerase and capsular polysaccharide when grown on glucose-containing media. Interrupted mating experiments to determine the kinetics of genetic transfer show that the two mannose-negative mutations map together between the histidine and tryptophan regions of the E. coli chromosome.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER H. I., HARDIGREE A. A. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:720–726. doi: 10.1128/jb.87.3.720-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADLER H. I., HARDIGREE A. A. POSTIRRADIATION GROWTH, DIVISION, AND RECOVERY IN BACTERIA. Radiat Res. 1965 May;25:92–102. [PubMed] [Google Scholar]
  3. HOWARD-FLANDERS P., SIMSON E., THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. doi: 10.1093/genetics/49.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  5. Kang S., Markovitz A. Induction of capsular polysaccharide synthesis by rho-fluorophenylalanine in Escherichia coli wild type and strains with altered phenylalanyl soluble ribonucleic acid synthetase. J Bacteriol. 1967 Feb;93(2):584–591. doi: 10.1128/jb.93.2.584-591.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MARKOVITZ A. REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1964 Feb;51:239–246. doi: 10.1073/pnas.51.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Markovitz A., Baker B. Suppression of radiation sensitivity and capsular polysaccharide synthesis in Escherichia coli K-12 by ochre suppressors. J Bacteriol. 1967 Aug;94(2):388–395. doi: 10.1128/jb.94.2.388-395.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Markovitz A., Lieberman M. M., Rosenbaum N. Derepression of phosphomannose isomerase by regulator gene mutations involved in capsular polysaccharide synthesis in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1497–1501. doi: 10.1128/jb.94.5.1497-1501.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Markovitz A., Rosenbaum N. A regulator gene that is dominant on an episome and recessive on a chromosome. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1084–1091. doi: 10.1073/pnas.54.4.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosen S. M., Zeleznick L. D., Fraenkel D., Wiener I. M., Osborn M. J., Horecker B. L. Characterization of the cell wall lipopolysaccharide of a mutant of Salmonella typhimurium lacking phosphomannose isomerase. Biochem Z. 1965 Aug 19;342(4):375–386. [PubMed] [Google Scholar]
  13. SAPELLI R. V., GOEBEL W. F. THE CAPSULAR POLYSACCHARIDE OF A MUCOID VARIANT OF E. COLI K 12. Proc Natl Acad Sci U S A. 1964 Aug;52:265–271. doi: 10.1073/pnas.52.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SOFFER R. L. Enzymatic expression of genetic units of function concerned with galactose metabolism in Escherichia coli. J Bacteriol. 1961 Oct;82:471–478. doi: 10.1128/jb.82.4.471-478.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shapiro J. A. Chromosomal location of the gene determining uridine diphoglucose formation in Escherichia coli K-12. J Bacteriol. 1966 Aug;92(2):518–520. doi: 10.1128/jb.92.2.518-520.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TAYLOR A. L., THOMAN M. S. THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964 Oct;50:659–677. doi: 10.1093/genetics/50.4.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taylor A L, Adelberg E A. Linkage Analysis with Very High Frequency Males of Escherichia Coli. Genetics. 1960 Sep;45(9):1233–1243. doi: 10.1093/genetics/45.9.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES