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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Dev Cell. 2009 Oct;17(4):482–493. doi: 10.1016/j.devcel.2009.07.016

Figure 2. MT2-MMP is predominantly expressed in the SMG epithelium and increases when branching morphogenesis begins.

Figure 2

(A) qPCR analysis of SMG gene expression at distinct developmental stages shows that MT1, MT2, and MT3 increase at E13 when branching morphogenesis begins.

(B) qPCR analysis of separated E13 SMG epithelium and mesenchyme shows that MT2 is predominant in the epithelium, whereas MT1 and MT3 are predominant in the mesenchyme. Error bars represent ± SD.

(C) Immunofluorescent analysis of MT-MMPs in E13 SMGs is in agreement with the qPCR data and shows that MT1 accumulates in the mesenchyme around the end buds and in the clefts, MT2 is localized throughout the epithelium, and MT3 is in both tissues. Perlecan, a BM marker (green), and Topro3, a nuclear stain (blue), are shown. M: mesenchyme; E: epithelium. Scale bar = 50µM.