Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1967 Nov;94(5):1679–1683. doi: 10.1128/jb.94.5.1679-1683.1967

Phospholipids of Thiobacillus thiooxidans

J M Shively 1, A A Benson 2
PMCID: PMC276878  PMID: 6066049

Abstract

Cells and spent growth media from sulfur- and thiosulfate-grown cultures of Thiobacillus thiooxidans were analyzed. The phosphatides were examined by thinlayer chromatography, and the products of their hydrolysis by hydrochloric acid and methanolic potassium hydroxide were separated by paper chromatography. The phospholipids in both cells and spent growth media were identified as phosphatidyl ethanolamine, phosphatidyl-N-monomethylethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. These comprised about 97% of the total lipid phosphorus. Lyso-phosphatidyl-N-monomethylethanolamine and lysophosphatidyl glycerol accounted for the remaining 3%. The percentage of the total lipid phosphorus accounted for by each phospholipid depended on the age of the culture.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENSON A. A., MARUO B. Piant phospholipids. I. Identification of the phosphatidyl glycerols. Biochim Biophys Acta. 1958 Jan;27(1):189–195. doi: 10.1016/0006-3002(58)90308-1. [DOI] [PubMed] [Google Scholar]
  2. BENSON A. A., STRICKLAND E. H. Plant phospholipids. 3. Identification of diphosphatidyl glycerol. Biochim Biophys Acta. 1960 Jul 1;41:328–333. doi: 10.1016/0006-3002(60)90016-0. [DOI] [PubMed] [Google Scholar]
  3. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  4. BREMER J., GREENBERG D. M. Mono- and dimethylethanolamine isolated from rat-liver phospholipids. Biochim Biophys Acta. 1959 Sep;35:287–288. doi: 10.1016/0006-3002(59)90375-0. [DOI] [PubMed] [Google Scholar]
  5. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. GOLDFINE H., ELLIS M. E. N-METHYL GROUPS IN BACTERIAL LIPIDS. J Bacteriol. 1964 Jan;87:8–15. doi: 10.1128/jb.87.1.8-15.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HUGHES D. E. The bacterial cytoplasmic membrane. J Gen Microbiol. 1962 Sep;29:39–46. doi: 10.1099/00221287-29-1-39. [DOI] [PubMed] [Google Scholar]
  10. Hagen P. O., Goldfine H., Williams P. J. Phospholipids of bacteria with extensive intracytoplasmic membranes. Science. 1966 Mar 25;151(3717):1543–1544. doi: 10.1126/science.151.3717.1543. [DOI] [PubMed] [Google Scholar]
  11. Houtsmuller U. M., van Deenen L. L. On the amino acid esters of phosphatidyl glycerol from bacteria. Biochim Biophys Acta. 1965 Dec 2;106(3):564–576. doi: 10.1016/0005-2760(65)90072-x. [DOI] [PubMed] [Google Scholar]
  12. Ikawa M. Bacterial phosphatides and natural relationships. Bacteriol Rev. 1967 Mar;31(1):54–64. doi: 10.1128/br.31.1.54-64.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JONES G. E., BENSON A. A. PHOSPHATIDYL GLYCEROL IN THIOBACILLUS THIOOXIDANS. J Bacteriol. 1965 Jan;89:260–261. doi: 10.1128/jb.89.1.260-261.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kates M. Bacterial lipids. Adv Lipid Res. 1964;2:17–90. [PubMed] [Google Scholar]
  15. Kates M., Volcani B. E. Lipid components of diatoms. Biochim Biophys Acta. 1966 Apr 4;116(2):264–278. doi: 10.1016/0005-2760(66)90009-9. [DOI] [PubMed] [Google Scholar]
  16. LASCELLES J., SZILAGYI J. F. PHOSPHOLIPID SYNTHESIS BY RHODOPSEUDOMONAS SPHEROIDES IN RELATION TO THE FORMATION OF PHOTOSYNTHETIC PIGMENTS. J Gen Microbiol. 1965 Jan;38:55–64. doi: 10.1099/00221287-38-1-55. [DOI] [PubMed] [Google Scholar]
  17. Letters R., Brown B. Phospholipids of yeast. I. Improvements in analysis by two-dimensional strip-transfer paper chromatography. Biochim Biophys Acta. 1966 Jun 1;116(3):482–488. [PubMed] [Google Scholar]
  18. Park C. E., Berger L. R. Complex lipids of Rhodomicrobium vannielii. J Bacteriol. 1967 Jan;93(1):221–229. doi: 10.1128/jb.93.1.221-229.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SCHAEFFER W. I., UMBREIT W. W. Phosphotidylinositol as a wetting agent in sulfur oxidation by Thiobacillus thiooxidans. J Bacteriol. 1963 Feb;85:492–493. doi: 10.1128/jb.85.2.492-493.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SHAPIRO D., RABINSOHN Y. SYNTHESIS OF N-MONOMETHYL- AND N,N-DIMETHYLCEPHALINS. Biochemistry. 1964 Apr;3:603–605. doi: 10.1021/bi00892a023. [DOI] [PubMed] [Google Scholar]
  21. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  22. VORBECK M. L., MARINETTI G. V. SEPARATION OF GLYCOSYL DIGLYCERIDES FROM PHOSPHATIDES USING SILICIC ACID COLUMN CHROMATOGRAPHY. J Lipid Res. 1965 Jan;6:3–6. [PubMed] [Google Scholar]
  23. Waksman S. A., Joffe J. S. Microörganisms Concerned in the Oxidation of Sulfur in the Soil: II. Thiobacillus Thiooxidans, a New Sulfur-oxidizing Organism Isolated from the Soil. J Bacteriol. 1922 Mar;7(2):239–256. doi: 10.1128/jb.7.2.239-256.1922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wood B. J., Nichols B. W., James A. T. The lipids and fatty acid metabolism of photosynthetic bacteria. Biochim Biophys Acta. 1965 Oct 4;106(2):261–273. doi: 10.1016/0005-2760(65)90034-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES