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. 1967 Dec;94(6):1846–1853. doi: 10.1128/jb.94.6.1846-1853.1967

Biosynthesis of Biotin in Microorganisms V. Control of Vitamer Production

Jerome Birnbaum a,1, Chik H Pai a,2, Herman C Lichstein a
PMCID: PMC276913  PMID: 4965364

Abstract

Use of a yeast-lactobacillus differential microbiological assay permitted investigation into the synthesis of biotin vitamers by a variety of bacteria. A major portion of the biotin activity was found extracellularly. The level of total biotin (assayable with yeast) greatly exceeded the level of true biotin (assayed with lactobacillus). Values for intracellular biotin generally showed good agreement between the assays, suggesting the presence of only true biotin within the cells. Bioautographic analysis of the medium after growth of each organism revealed the presence of large amounts of a vitamer which corresponded to dl-desthiobiotin on the basis of Rf value and biological activity. Biotin, when detected at all, was at very low concentrations. Also, an avidin-uncombinable vitamer was synthesized by a majority of the bacteria. Addition of d-biotin to the growth medium prevented completely the synthesis of both vitamers of biotin. d-Biotin-d-sulfoxide had no effect on the synthesis of desthiobiotin or the avidin-uncombinable vitamer. Addition of dl-desthiobiotin did not prevent its own synthesis nor that of the other vitamer. Control of vitamer synthesis is therefore highly specific for d-biotin. The avidin-uncombinable vitamer was produced only at repressed levels in the presence of high concentrations of both d-biotin and dl-desthiobiotin, which suggested that it is not a degradation product of these substances. A possible mechanism for the overproduction of the biosynthetic precursors of biotin is presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIRNBAUM J., LICHSTEIN H. C. CONVERSION OF D-BIOTIN TO BIOTIN VITAMERS BY LACTOBACILLUS ARABINOSUS. J Bacteriol. 1965 Apr;89:1035–1040. doi: 10.1128/jb.89.4.1035-1040.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DHYSE F. G., HERTZ R. The effects of actithiazic acid on egg white-induced biotin deficiency and upon the microbial formation of biotin vitamers in the rat. Arch Biochem Biophys. 1958 Mar;74(1):7–16. doi: 10.1016/0003-9861(58)90194-2. [DOI] [PubMed] [Google Scholar]
  3. EISENBERG M. A. BIOTIN BIOSYNTHESIS. I. BIOTIN YIELDS AND BIOTIN VITAMERS IN CULTURES OF PHYCOMYCES BLAKESLEEANUS. J Bacteriol. 1963 Oct;86:673–680. doi: 10.1128/jb.86.4.673-680.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisenberg M. A., Maseda R. An early intermediate in the biosynthesis of biotin: Incorporation studies with [1,7-C(2)]pimelic acid. Biochem J. 1966 Dec;101(3):601–606. doi: 10.1042/bj1010601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GENGHOF D. S. Production of vitamers of biotin by Corynebacterium xerose. Arch Biochem Biophys. 1956 May;62(1):63–69. doi: 10.1016/0003-9861(56)90087-x. [DOI] [PubMed] [Google Scholar]
  6. Leonian L. H., Lilly V. G. Conversion of Desthiobiotin Into Biotin or Biotinlike Substances by Some Microorganisms. J Bacteriol. 1945 Mar;49(3):291–297. doi: 10.1128/jb.49.3.291-297.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MELVILLE D. B., GENGHOF D. S., LEE J. M. Biological properties of biotin d- and l-sulfoxides. J Biol Chem. 1954 Jun;208(2):503–512. [PubMed] [Google Scholar]
  8. MOYED H. S. Interference with the feed-back control of histidine biosynthesis. J Biol Chem. 1961 Aug;236:2261–2267. [PubMed] [Google Scholar]
  9. PAI C. H., LICHSTEIN H. C. Control of biotin synthesis in Escherichia coli by exogenous biotin. Biochim Biophys Acta. 1962 Nov 19;65:159–160. doi: 10.1016/0006-3002(62)90165-8. [DOI] [PubMed] [Google Scholar]
  10. PAI C. H., LICHSTEIN H. C. THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. 3. THE BIOSYNTHESIS OF (+)-BIOTIN FROM DESTHIOBIOTIN AND ITS CONTROL IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 12;100:43–49. doi: 10.1016/0304-4165(65)90425-3. [DOI] [PubMed] [Google Scholar]
  11. PAI C. H., LICHSTEIN H. C. THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. I. THE PHYSIOLOGY OF BIOTIN SYNTHESIS IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 12;100:28–35. doi: 10.1016/0304-4165(65)90423-x. [DOI] [PubMed] [Google Scholar]
  12. PAI C. H., LICHSTEIN H. C. THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. II. MECHANISM OF THE REGULATION OF BIOTIN SYNTHESIS IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 Apr 12;100:36–42. doi: 10.1016/0304-4165(65)90424-1. [DOI] [PubMed] [Google Scholar]
  13. Pai C. H., Lichstein H. C. Biosynthesis of biotin in microorganisms. IV. Repression and derepression of (+ -)-biotin synthesis from (+)-desthiobiotin. Arch Biochem Biophys. 1966 Apr;114(1):138–144. doi: 10.1016/0003-9861(66)90314-6. [DOI] [PubMed] [Google Scholar]
  14. Tepper J. P., McCormick D. B., Wright L. D. Direct evidence for the conversion of dethiobiotin to biotin in Aspergillus niger. J Biol Chem. 1966 Dec 10;241(23):5734–5735. [PubMed] [Google Scholar]
  15. WRIGHT L. D., CRESSON E. L., DRISCOLL C. A. Bioautography of biotin and certain related compounds. Proc Soc Exp Biol Med. 1954 Jul;86(3):480–483. doi: 10.3181/00379727-86-21140. [DOI] [PubMed] [Google Scholar]

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