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. 1967 Dec;94(6):1854–1867. doi: 10.1128/jb.94.6.1854-1867.1967

Extraction, Characterization, and Cellular Localization of the Lipids of Staphylococcus aureus

David C White 1, Frank E Frerman 1
PMCID: PMC276914  PMID: 4965365

Abstract

Satisfactory extraction and assay procedures have been developed for the lipids of Staphylococcus aureus. The following lipids have been characterized in detail: the vitamin K2, which is shown to exist as isoprenologues with side chains of 35, 40, and 45 carbon atoms; monoglucosyldiglyceride and diglucosyldiglyceride, which account for all the carbohydrate in the lipid extracts; the lysyl ester of phosphatidyl glycerol, phosphatidyl glycerol, and cardiolipin, which account for 98% of the phosphate in the lipid extract. The extraction procedure removes 98% of the total bacterial fatty acids. Acidification of the medium before harvest and refluxing in isopropanol are critical in the extraction procedure for the maximal recovery of lysyl-phosphatidyl glycerol and the glucolipids. The lipids have been shown to be a part of the same membrane as the respiratory pigments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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