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. 1967 Dec;94(6):1924–1929. doi: 10.1128/jb.94.6.1924-1929.1967

Effect of Nutrients on Physiological Properties of Clostridium botulinum Type E

B Gullmar 1, N Molin 1
PMCID: PMC276923  PMID: 4864406

Abstract

Eight strains of Clostridium botulinum type E out of twelve tested showed good growth and normal cell morphology in a synthetic medium containing choline. Growth and toxin production by a representative strain was not influenced by repeated subculturing. In the chemically defined medium, acetylcholine, N,N-dimethylethanolamine, and lecithin could replace choline to get normal cell division and cell morphology of C. botulinum type E. Choline could not be replaced by ethanolamine, N-methylethanolamine, or betaine. A toxigenic strain of C. botulinum type E showed proteolytic, lipolytic, and lecithinase activity in complex media but not in a synthetic medium. On prolonged incubation in the high temperature range of growth, the toxicity of the culture filtrate decreased in a complex, but not in a synthetic medium. The implications of these findings are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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