Abstract
Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97–103. 1964.—A factor present in plant and animal sources was found necessary for the incorporation of formate-C14 into pyruvate by Streptococcus faecalis 10Cl. Yeast extract produced a response linear in the range between 10 and 30 mg/ml of reaction mixture. Soy peptone, beef peptone, and Brain Heart Infusion replaced yeast extract, but various intermediates, cofactors, amino acids, purines, pyrimidines, and peptides did not stimulate the reaction. A lag occurred in the rate of formate incorporation that was not influenced by anaerobic conditions or growth of cells in a medium containing pyruvate and formate. Phosphate or maleate buffer permitted rapid exchange velocities but tris(hydroxymethyl)aminomethane or collidine buffer was inhibitory. Heating yeast extract at 121 C for 15 min in 3 n H2SO4 produced 66% inactivation of the factor(s), whereas treatment with 3 n KOH produced 97% inactivation. The factor(s) was insoluble in butanol, benzene, ethyl acetate, or chloroform. The material adsorbed on Dowex-1 (OH−) and Amberlite IR-120 (H+) but not on Amberlite IR-4B (OH−). The active component(s) was highly polar, nonvolatile, dialyzable, and had amphoteric properties.
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Selected References
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- CHANTRENNE H., LIPMANN F. Coenzyme A dependence and acetyl donor function of the pyruvate-formate exchange system. J Biol Chem. 1950 Dec;187(2):757–767. [PubMed] [Google Scholar]
- GALE E. F. The assimilation of amino-acids by bacteria; action of inhibitors on the accumulation of free glutamic acid in Staphylococcus aureus and Streptococcus faecalis. Biochem J. 1951 Mar;48(3):286–290. doi: 10.1042/bj0480286. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GUNSALUS I. C., DOLIN M. I., STRUGLIA L. Pyruvic acid metabolism. III. A manometric assay for pyruvate oxidation factor. J Biol Chem. 1952 Feb;194(2):849–857. [PubMed] [Google Scholar]
- McCormick N. G., Ordal E. J., Whiteley H. R. DEGRADATION OF PYRUVATE BY MICROCOCCUS LACTILYTICUS II. : Studies of Cofactors in the Formate-Exchange Reaction. J Bacteriol. 1962 Apr;83(4):899–906. doi: 10.1128/jb.83.4.899-906.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOVELLI G. D. The exchange of H14COOH with the carboxyl group of pyruvate by Clostridium butylicum and Micrococcus lactilyticus. Biochim Biophys Acta. 1955 Dec;18(4):594–596. doi: 10.1016/0006-3002(55)90170-0. [DOI] [PubMed] [Google Scholar]
- O'kane D. J., Gunsalus I. C. Pyruvic Acid Metabolism: A Factor Required for Oxidation by Streptococcus faecalis. J Bacteriol. 1948 Oct;56(4):499–506. doi: 10.1128/jb.56.4.499-506.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OSTER M. O., WOOD N. P. FORMATE--PYRUVATE EXCHANGE REACTION IN STREPTOCOCCUS FAECALIS. II. REACTION CONDITIONS FOR CELL EXTRACTS. J Bacteriol. 1964 Jan;87:104–113. doi: 10.1128/jb.87.1.104-113.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STRECKER H. J. Formate fixation in pyruvate by Escherichia coli. J Biol Chem. 1951 Apr;189(2):815–830. [PubMed] [Google Scholar]