Abstract
Walker, D. J. (CSIRO, University of Adelaide, Adelaide, South Australia), and W. W. Forrest. Endogenous metabolism in Streptococcus faecalis. J. Bacteriol. 87:256–262. 1964.—Washed suspensions of Streptococcus faecalis incubated in phosphate buffer (pH 6.0) under anaerobic conditions released amino acids into the suspending medium. Little or no carbohydrate was released in soluble form, and no acid production could be detected. Cell lipid was stable, and only a small amount of nucleotide (material absorbing at 260 mμ) was released. Estimates of total carbon produced in soluble form showed that this could be almost entirely accounted for as amino acid. Over the short period during which amino acid was produced (up to 5 hr), the glycolytic activity of the cells remained fairly constant, and fell slowly thereafter to low values in 24 to 48 hr; the rate of fall depended on cell concentration. Whereas exogenous sources of energy protected against loss of glycolytic activity, arsenate, hydroxylamine, or replacement of phosphate by other buffers accelerated loss of glycolytic activity. Restoration of glycolytic activity in aged cells could be achieved by incubation with amino acids plus an energy source, with concurrent synthesis of protein but no apparent growth. Ammonium ion was not effective in protecting or restoring glycolytic activity. The nature of changes which took place during endogenous metabolism are discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Conway E. J., O'malley E. Microdiffusion methods. Ammonia and urea using buffered absorbents (revised methods for ranges greater than 10mug. N). Biochem J. 1942 Sep;36(7-9):655–661. doi: 10.1042/bj0360655. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAWES E. A., RIBBONS D. W. The endogenous metabolism of microorganisms. Annu Rev Microbiol. 1962;16:241–264. doi: 10.1146/annurev.mi.16.100162.001325. [DOI] [PubMed] [Google Scholar]
- FORREST W. W., WALKER D. J., HOPGOOD M. F. Enthalpy changes associated with the lactic fermentation of glucose. J Bacteriol. 1961 Nov;82:685–690. doi: 10.1128/jb.82.5.685-690.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRONLUND A. F., CAMPBELL J. J. Nitrogenous compounds as substrates for endogenous respiration in microorganisms. J Bacteriol. 1961 May;81:721–724. doi: 10.1128/jb.81.5.721-724.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALLIWELL G. A micro-determination of carbohydrates and proteins. Biochem J. 1960 Mar;74:457–462. doi: 10.1042/bj0740457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KNIVETT V. A. Phosphorylation coupled with anaerobic breakdown of citrulline. Biochem J. 1954 Apr;56(4):602–606. [PMC free article] [PubMed] [Google Scholar]
- Markham R. A steam distillation apparatus suitable for micro-Kjeldahl analysis. Biochem J. 1942 Dec;36(10-12):790–791. doi: 10.1042/bj0360790. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson W. V. THE PREPARATION OF SODIUM PYRUVATE. Science. 1942 Jul 24;96(2482):93–94. doi: 10.1126/science.96.2482.93. [DOI] [PubMed] [Google Scholar]
- TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WADE H. E., MORGAN D. M. The nature of the fluctuating ribonucleic acid in Escherichia coli. Biochem J. 1957 Feb;65(2):321–331. doi: 10.1042/bj0650321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZARLENGO M., ABRAMS A. Selective penetration of ammonia and alkylamines into Streptococcus fecalis and their effect on glycolysis. Biochim Biophys Acta. 1963 Apr 2;71:65–77. doi: 10.1016/0006-3002(63)90986-7. [DOI] [PubMed] [Google Scholar]