Abstract
Okrend, Harold (Rutgers, The State University, New Brunswick, N.J.), and Norman C. Dondero. Requirement of Sphaerotilus for cyanocobalamin. J. Bacteriol. 87:286–292. 1964.—Cultures of Sphaerotilus grew well in a culture medium containing pancreatic digest of casein, glycerol, and yeast autolysate, but grew poorly in a defined medium containing monosodium glutamate, glycerol, and salts. Measurement of dry weight, cell nitrogen, and deoxyribonucleic acid showed that the addition of 1.0 mμg/ml of cyancobalamin to the defined medium gave growth equivalent to that obtained with the pancreatic digest of casein. Methionine, in a concentration of 2 × 104-fold, relieved the requirement for cyanocobalamin, but cobalt did not replace cyanocobalamin nor stimulate growth. Of 38 additional cultures of diverse origin, 19 showed stimulation by visible comparison of growth in tubes; nonquantitative comparisons, however, were not reliable for showing differences in growth of this organism.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barker H. A., Weissbach H., Smyth R. D. A COENZYME CONTAINING PSEUDOVITAMIN B(12). Proc Natl Acad Sci U S A. 1958 Nov 15;44(11):1093–1097. doi: 10.1073/pnas.44.11.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DONDERO N. C., PHILLIPS R. A., HEUKELEKIAN H. Isolation and preservation of cultures of Sphaerotilus. Appl Microbiol. 1961 May;9:219–227. doi: 10.1128/am.9.3.219-227.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ECKER R. E., LOCKHART W. R. Specific effect of limiting nutrient on physiological events during culture growth. J Bacteriol. 1961 Oct;82:511–516. doi: 10.1128/jb.82.4.511-516.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOHNL G. Ernährungs- und stoffwechselphysiologische Untersuchungen an Sphaerotilus natans. Arch Mikrobiol. 1955;23(3):207–250. [PubMed] [Google Scholar]
- MULDER E. G., VAN VEENW INVESTIGATIONS ON THE SPHAEROTILUSLEPTOTHRIX GROUP. Antonie Van Leeuwenhoek. 1963;29:121–153. doi: 10.1007/BF02046045. [DOI] [PubMed] [Google Scholar]
- STOKES J. L. Studies on the filamentous sheathed iron bacterium Sphaerotilus natans. J Bacteriol. 1954 Mar;67(3):278–291. doi: 10.1128/jb.67.3.278-291.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]