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. 1964 Feb;87(2):323–328. doi: 10.1128/jb.87.2.323-328.1964

RIBONUCLEIC ACIDS OF BACILLUS SUBTILIS SPORES AND SPORULATING CELLS

Roy H Doi 1, Richard T Igarashi 1
PMCID: PMC277010  PMID: 14151051

Abstract

Doi, Roy H. (Syracuse University, Syracuse N.Y.), and Richard T. Igarashi. Ribonucleic acids of Bacillus subtilus spores and sporulating cells. J. Bacteriol. 87:323–328. 1964.—The ribonucleic acids (RNA) of Bacillus subtilis spores were analyzed for their size and base composition. Soluble and ribosomal RNA identical to those found in vegetative cells were present in spores. A base ratio difference was observed between the 16s and 23s ribosomal RNA. The 16s RNA had a higher cytidylate and lower adenylate base composition. The ratio of soluble of ribosomal RNA in spores was 30%, in contrast to 15% in vegetative cells, and may be related to their difference in biosynthetic activity. Although active synthesis of informational RNA occurred prior to endospore formation, no detectable level of informational RNA was found in spores. The informational RNA was shown to have the same base composition as that of B. subtilis deoxyribonucleic acid. The compositional difference between spore and vegetative cell RNA appeared to be strictly quantitative.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALASSA G. [Ribonucleic acid from Bacillus subtilis spores]. Biochim Biophys Acta. 1963 Jul 30;72:497–500. doi: 10.1016/0006-3002(63)90272-5. [DOI] [PubMed] [Google Scholar]
  2. DEL VALLE M. R., ARONSON A. I. Evidence for the synthesis of stable informational RNA required for bacterial spore formation. Biochem Biophys Res Commun. 1962 Nov 27;9:421–425. doi: 10.1016/0006-291x(62)90027-x. [DOI] [PubMed] [Google Scholar]
  3. GIERER A., SCHRAMM G. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature. 1956 Apr 14;177(4511):702–703. doi: 10.1038/177702a0. [DOI] [PubMed] [Google Scholar]
  4. GREEN M. H., HALL B. D. A comparison of the native and derived 30S and 50S ribosomes of Escherichia coli. Biophys J. 1961 Jul;1:517–523. doi: 10.1016/s0006-3495(61)86905-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HAYASHI M., SPIEGELMAN S. The selective synthesis of informational RNA in bacteria. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1564–1580. doi: 10.1073/pnas.47.10.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  7. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  8. Marmur J., Seaman E., Levine J. INTERSPECIFIC TRANSFORMATION IN BACILLUS. J Bacteriol. 1963 Feb;85(2):461–467. doi: 10.1128/jb.85.2.461-467.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. POWELL J. F. Isolation of dipicolinic acid (pyridine-2:6-dicarboxylic acid) from spores of Bacillus megatherium. Biochem J. 1953 May;54(2):210–211. doi: 10.1042/bj0540210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SACKS L. E., ALDERTON G. Behavior of bacterial spores in aqueous polymer two-phase systems. J Bacteriol. 1961 Sep;82:331–341. doi: 10.1128/jb.82.3.331-341.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. WOESE C. R., FORRO J. R. Correlations between ribonucleic acid and deoxyribonucleic acid metabolism during spore germination. J Bacteriol. 1960 Dec;80:811–817. doi: 10.1128/jb.80.6.811-817.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WOESE C. R. Unusual ribosome particles occurring during spore germination. J Bacteriol. 1961 Nov;82:695–701. doi: 10.1128/jb.82.5.695-701.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. YANKOFSKY S. A., SPIEGELMAN S. Distinct cistrons for the two ribosomal RNA components. Proc Natl Acad Sci U S A. 1963 Apr;49:538–544. doi: 10.1073/pnas.49.4.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. YOUNG I. E., FITZ-JAMES P. C. Chemical and morphological studies of bacterial spore formation. II. Spore and parasporal protein formation in Bacillus cereus var. alesti. J Biophys Biochem Cytol. 1959 Dec;6:483–498. doi: 10.1083/jcb.6.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]

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