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. 1964 Apr;87(4):828–831. doi: 10.1128/jb.87.4.828-831.1964

PREFERENTIAL SYNTHESIS OF α-AMYLASE BY BACILLUS STEAROTHERMOPHILUS IN THE PRESENCE OF 5-METHYL-TRYPTOPHAN1

N E Welker 1,2,2, L Leon Campbell 1,2,2
PMCID: PMC277100  PMID: 14137620

Abstract

Welker, N. E. (Western Reserve University, Cleveland, Ohio), and L. Leon Campbell. Preferential synthesis of α-amylase by Bacillus stearothermophilus in the presence of 5-methyl-tryptophan. J. Bacteriol. 87:828–831. 1964.—Washed-cell suspensions of Bacillus stearothermophilus induced with pure maltose preferentially synthesized α-amylase in the presence of 5-methyl-tryptophan (5-MT). 5-MT did not inhibit the formation of active α-amylase or the incorporation of proline-C14 into α-amylase. In contrast, p-fluorophenylalanine inhibited the formation of active α-amylase by 92% and the incorporation of proline-C14 into the enzyme by 95%. p-Fluorophenylalanine and 5-MT inhibited cellular protein synthesis, as measured by proline-C14 incorporation, by 74 and 72%, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CAMPBELL L. L., MANNING G. B. Thermostable alpha-amylase of Bacillus stearothermophilus. III. Amino acid composition. J Biol Chem. 1961 Nov;236:2962–2965. [PubMed] [Google Scholar]
  2. EISENSTADT J. M., KLEIN H. P. Selective formation of alpha-amylase by non-growing cells of Pseudomonas saccharophila. Biochim Biophys Acta. 1960 Oct 21;44:206–208. doi: 10.1016/0006-3002(60)91554-7. [DOI] [PubMed] [Google Scholar]
  3. MOYED H. S. False feedback inhibition: inhibition of tryptophan biosynthesis by 5-methyltryptophan. J Biol Chem. 1960 Apr;235:1098–1102. [PubMed] [Google Scholar]
  4. RICHMOND M. H. The effect of amino acid analogues on growth and protein synthesis in microorganisms. Bacteriol Rev. 1962 Dec;26:398–420. doi: 10.1128/br.26.4.398-420.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. RICKENBERG H. V., LESTER G. The preferential synthesis of beta-galactosidase in Escherichia coli. J Gen Microbiol. 1955 Oct;13(2):279–284. doi: 10.1099/00221287-13-2-279. [DOI] [PubMed] [Google Scholar]
  6. TRUDINGER P. A., COHEN G. N. The effect of 4-methyltryptophan on growth and enzyme systems of Escherichia coli. Biochem J. 1956 Mar;62(3):488–491. doi: 10.1042/bj0620488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. TURNOCK G., WILD D. G. Accumulation of ribonucleic acid in Escherichia coli due to the action of 5-methyl tryptophan. Nature. 1963 Feb 9;197:597–598. doi: 10.1038/197597a0. [DOI] [PubMed] [Google Scholar]
  8. WELKER N. E., CAMPBELL L. L. EFFECT OF CARBON SOURCES ON FORMATION OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS. J Bacteriol. 1963 Oct;86:681–686. doi: 10.1128/jb.86.4.681-686.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. WELKER N. E., CAMPBELL L. L. INDUCED BIOSYNTHESIS OF ALPHA-AMYLASE BY GROWING CULTURES OF BACILLUS STEAROTHERMOPHILUS. J Bacteriol. 1963 Dec;86:1196–1201. doi: 10.1128/jb.86.6.1196-1201.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. WELKER N. E., CAMPBELL L. L. INDUCTION OF ALPHA-AMYLASE OF BACILLUS STEAROTHERMOPHILUS BY MALTODEXTRINS. J Bacteriol. 1963 Oct;86:687–691. doi: 10.1128/jb.86.4.687-691.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. YANAGISAWA K. Preferential synthesis of beta-galactosidase after amino acid starvation. Biochem Biophys Res Commun. 1962 Sep 25;9:84–87. doi: 10.1016/0006-291x(62)90092-x. [DOI] [PubMed] [Google Scholar]

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