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. 1964 Apr;87(4):945–951. doi: 10.1128/jb.87.4.945-951.1964

POROSITY OF ISOLATED CELL WALLS OF SACCHAROMYCES CEREVISIAE AND BACILLUS MEGATERIUM

Philipp Gerhardt 1, Jean A Judge 1
PMCID: PMC277115  PMID: 14137635

Abstract

Gerhardt, Philipp (The University of Michigan, Ann Arbor), and Jean A. Judge. Porosity of isolated cell walls of a yeast and a bacillus. J. Bacteriol. 87:945–951. 1964.—Decagram masses of cell walls were isolated from Saccharomyces cerevisiae and Bacillus megaterium; their porosity was examined by measuring the extent of uptake with polyethylene glycols and dextrans varying in molecular weight from 62 to 2,000,000. The results indicated that both walls are heteroporous. The near equality of extrapolated water-uptake values and determined moisture contents suggested that water in the cell walls is mainly free for distribution of solutes. Polymers with molecular weights of 4,500 and above were excluded by the yeast walls, and those with molecular weights of 57,000 were excluded by the bacillus walls; from these results, maximal openings of 36 and 107 A, respectively, were calculated. Electron micrographs of shadowed, stained, and sectioned walls revealed fine structure not inconsistent with heteroporosity, but the predicted openings were not seen. Altogether, in structure and permeability behavior, the cell walls were like a random meshwork of cross-linked macromolecular strands.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTHOLOMEW J. W., LEVIN R. The structure of Saccharomyces carlsbergensis and S. cerevisiae as determined by ultra-thin sectioning methods and electron microscopy. J Gen Microbiol. 1955 Jun;12(3):473–477. doi: 10.1099/00221287-12-3-473. [DOI] [PubMed] [Google Scholar]
  2. BLACK S. H., GERHARDT P. Permeability of bacterial spores. IV. Water content, uptake, and distribution. J Bacteriol. 1962 May;83:960–967. doi: 10.1128/jb.83.5.960-967.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRITT E. M., GERHARDT P. Bacterial permeability; total uptake of lysine by intact cells, protoplasts, and cell walls of Micrococcus lysodeikticus. J Bacteriol. 1958 Sep;76(3):288–293. doi: 10.1128/jb.76.3.288-293.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONWAY E. J., DOWNEY M. An outer metabolic region of the yeast cell. Biochem J. 1950 Sep;47(3):347–355. doi: 10.1042/bj0470347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GROTTE G. Passage of dextran molecules across the blood-lymph barrier. Acta Chir Scand Suppl. 1956;211:1–84. [PubMed] [Google Scholar]
  6. HOUWINK A. L., KREGER D. R. Observations on the cell wall of yeasts; an electron microscope and x-ray diffraction study. Antonie Van Leeuwenhoek. 1953;19(1):1–24. doi: 10.1007/BF02594830. [DOI] [PubMed] [Google Scholar]
  7. NICKERSON W. J. SYMPOSIUM ON BIOCHEMICAL BASES OF MORPHOGENESIS IN FUNGI. IV. MOLECULAR BASES OF FORM IN YEASTS. Bacteriol Rev. 1963 Sep;27:305–324. doi: 10.1128/br.27.3.305-324.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. NORTHCOTE D. H., HORNE R. W. The chemical composition and structure of the yeast cell wall. Biochem J. 1952 May;51(2):232–236. doi: 10.1042/bj0510232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PHAFF H. J. CELL WALL OF YEASTS. Annu Rev Microbiol. 1963;17:15–30. doi: 10.1146/annurev.mi.17.100163.000311. [DOI] [PubMed] [Google Scholar]
  10. RIBI E., PERRINE T., LIST R., BROWN W., GOODE G. Use of pressure cell to prepare cell walls from Mycobacteria. Proc Soc Exp Biol Med. 1959 Mar;100(3):647–649. doi: 10.3181/00379727-100-24730. [DOI] [PubMed] [Google Scholar]
  11. ROGERS H. J. THE BACTERIAL CELL WALL. THE RESULT OF ADSORPTION, STRUCTURE OR SELECTIVE PERMEABILITY? J Gen Microbiol. 1963 Jul;32:19–24. doi: 10.1099/00221287-32-1-19. [DOI] [PubMed] [Google Scholar]
  12. SEIFTER S., DAYTON S. The estimation of glycogen with the anthrone reagent. Arch Biochem. 1950 Jan;25(1):191–200. [PubMed] [Google Scholar]
  13. SHAFFER C. B., CRITCHFIELD F. H., NAIR J. H., 3rd Chemical and physical examination of some polyethylene glycols. J Am Pharm Assoc Am Pharm Assoc. 1950 Jun;39(6):344–348. doi: 10.1002/jps.3030390614. [DOI] [PubMed] [Google Scholar]
  14. VITOLS E., NORTH R. J., LINNANE A. W. Studies on the oxidative metabolism of Saccharomyces cerevisiae. I. Observations on the fine structure of the yeast cell. J Biophys Biochem Cytol. 1961 Mar;9:689–699. doi: 10.1083/jcb.9.3.689. [DOI] [PMC free article] [PubMed] [Google Scholar]

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