Abstract
Martin, William J. (College of Medicine, University of Utah, Salt Lake City), and Stanley Marcus. Relation of pyrogenic and emetic properties of enterobacteriaceal endotoxin and of staphylococcal enterotoxin. J. Bacteriol. 87:1019–1026. 1964.—The pyrogenic, endotoxic fraction of enterobacteriaceal organisms, isolated in a relatively impure or purified state, is both pyrogenic in rabbits and cats as well as emeticogenic in cats in microgram amounts. Employing classical methods for preparation of enterobacteriaceal endotoxin from known enterotoxic strains of Staphylococcus aureus yielded preparations with less than one-hundredth the activity of the enterobacteriaceal material. However, a purified enterotoxin material from an S. aureus strain, with protein-like rather than polysaccharidelike properties, was found to be both pyrogenic and emeticogenic in microgram amounts. These results suggest the probability that the enterobacteriaceal pyrogenemetic substance(s) is a significant part of the whole cell, whereas the staphylococcal enterotoxin is elaborated as a metabolic product which apparently does not accumulate in the whole cell.
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Selected References
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- BERGDOLL M. S., SUGIYAMA H., DACK G. M. Staphylococcal enterotoxin. I. Purification. Arch Biochem Biophys. 1959 Nov;85:62–69. doi: 10.1016/0003-9861(59)90447-3. [DOI] [PubMed] [Google Scholar]
- DENNY C. B., BOHRER C. W. IMPROVED CAT TEST FOR ENTEROTOXIN. J Bacteriol. 1963 Aug;86:347–348. doi: 10.1128/jb.86.2.347-348.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDWARDS P. R. Preparation of antisera for detection of the somatic antigens of Salmonella cultures. Public Health Rep. 1951 Jun 29;66(26):837–839. [PubMed] [Google Scholar]
- HIBNICK H. E., BERGDOLL M. S. Staphylococcal enterotoxin. II. Chemistry. Arch Biochem Biophys. 1959 Nov;85:70–73. doi: 10.1016/0003-9861(59)90448-5. [DOI] [PubMed] [Google Scholar]
- Hammon W. M. Staphylococcus Enterotoxin: An Improved Cat Test, Chemical and Immunological Studies. Am J Public Health Nations Health. 1941 Nov;31(11):1191–1198. doi: 10.2105/ajph.31.11.1191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEENE W. R., SILBERMAN H. R., LANDY M. Observations on the pyrogenic response and its application to the bioassay of endotoxin. J Clin Invest. 1961 Feb;40:295–301. doi: 10.1172/JCI104256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARCUS S., ANSELMO C., PERKINS J. J. Studies on bacterial pyrogenicity. I. Quantitative basis. Proc Soc Exp Biol Med. 1958 Nov;99(2):359–362. doi: 10.3181/00379727-99-24350. [DOI] [PubMed] [Google Scholar]
- SUGIYAMA H., CHOW K. L., DRAGSTEDT L. R., 2nd Study of emetic receptor sites for staphylococcal enterotoxin in monkeys. Proc Soc Exp Biol Med. 1961 Oct;108:92–95. doi: 10.3181/00379727-108-26855. [DOI] [PubMed] [Google Scholar]