Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 May;87(5):1105–1113. doi: 10.1128/jb.87.5.1105-1113.1964

HETEROGENEITY OF APPARENTLY COMPLETE POLIOVIRUS PARTICLES

Joseph J Tumilowicz 1, Klaus Hummeler 1
PMCID: PMC277153  PMID: 4289441

Abstract

Tumilowicz, Joseph J. (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Klaus Hummeler. Heterogeneity of apparently complete poliovirus particles. J. Bacteriol. 87:1105–1113. 1964.—A chromatographic procedure was developed for separating the N from the H complement-fixing antigen of poliovirus. This procedure concomitantly effected some separation of classes of N particle. The ratio of physical virus particles (PVP) to plaque-forming units (PFU) in N-reactive fractions varied from 6 to 51. Fractions with the lowest PVP-PFU ratios represented a considerable enrichment of PFU, when compared with the original ratio in each experiment. A direct relationship was found between the ratio of complement-fixing units of N antigen [CFU(N)] to 1010 PVP and the ratio of PFU to 1010 PVP for most of the N-reactive fractions. Large differences among the PVP-CFU(N) ratios, along with relatively constant PFU-CFU(N) ratios for most of the N fractions, indicated that N antigen is not distributed equally among non-H particles. Two possibilities, both compatible with the results, were discussed for the manner in which N antigen might be distributed. An absolute value of 107 PVP-CFU(N) was proposed for particles with a PVP-PFU ratio of 1.

Full text

PDF
1105

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HOYER B. H., BOLTON E. T., ORMSBEE R. A., LEBOUVIER G., RITTER D. B., LARSON C. L. Mammalian viruses and Rickettsiae; their purification and recovery by cellulose anion exchange columns has significant implications. Science. 1958 Apr 18;127(3303):859–863. doi: 10.1126/science.127.3303.859. [DOI] [PubMed] [Google Scholar]
  2. HUMMELER K., ANDERSON T. F., BROWN R. A. Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology. 1962 Jan;16:84–90. doi: 10.1016/0042-6822(62)90205-2. [DOI] [PubMed] [Google Scholar]
  3. HUMMELER K., HAMPARIAN V. V. Studies on the complement fixing antigens of poliomyelitis. I. Demonstration of type and group specific antigens in native and heated viral preparations. J Immunol. 1958 Dec;81(6):499–505. [PubMed] [Google Scholar]
  4. HUMMELER K. Mumps complement fixing antibodies in guinea pigs. J Immunol. 1957 Oct;79(4):337–341. [PubMed] [Google Scholar]
  5. HUMMELER K., TUMILOWICZ J. J. Studies on the complement-fixing antigens of poliomylitis. II. Preparation of type-specific anti-N and anti-H indicator sera. J Immunol. 1960 Jun;84:630–634. [PubMed] [Google Scholar]
  6. JOKLIK W. K., DARNELL J. E., Jr The adsorption and early fate of purified poliovirus in HeLa cells. Virology. 1961 Apr;13:439–447. doi: 10.1016/0042-6822(61)90275-6. [DOI] [PubMed] [Google Scholar]
  7. MAYER M. M., RAPP H. J., ROIZMAN B., KLEIN S. W., COWAN K. M., LUKENS D. The purification of poliomyelitis virus as studied by complement fixation. J Immunol. 1957 Jun;78(6):435–455. [PubMed] [Google Scholar]
  8. ROIZMAN B., MAYER M. M., RAPP H. J. Immunochemical studies of poliovirus. III. Further studies on the immunologic and physical properties of poliovirus particles produced in tissue culture. J Immunol. 1958 Nov;81(5):419–425. [PubMed] [Google Scholar]
  9. TAYLOR J., GRAHAM A. F. Analysis of a plaque assay method for purified poliovirus MEF-1. Virology. 1961 Apr;13:427–438. doi: 10.1016/0042-6822(61)90274-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES