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. 1964 May;87(5):1123–1128. doi: 10.1128/jb.87.5.1123-1128.1964

GLUCOSAMINE-6-PHOSPHATE DEGRADATION BY PROTEUS VULGARIS AND ISOLATION OF PHOSPHOGLUCOSAMINISOMERASE1

H Aron a, H H Yonenaka a, H I Nakada a
PMCID: PMC277156  PMID: 5874535

Abstract

Aron, H. (University of California, Santa Barbara), H. H. Yonenaka, and H. I. Nakada. Glucosamine-6-phosphate degradation by Proteus vulgaris and isolation of phosphoglucosaminisomerase. J. Bacteriol. 87:1123–1128. 1964.—The presence of the enzyme, phosphoglucosaminisomerase, was demonstrated in a strain of Proteus vulgaris. Fructose-6-phosphate and ammonia were shown to be the primary reaction products. The optimal pH was 7.2 with a broad peak. This differs from previously reported bacterial phosphoglucosaminisomerases which had a pH optimum of about 5.8. Other properties of the enzyme are presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN D. H. The phosphorylation of D (+) glucosamine by crystalline yeast hexokinase. Biochim Biophys Acta. 1951 Nov;7(4):487–493. doi: 10.1016/0006-3002(51)90063-7. [DOI] [PubMed] [Google Scholar]
  2. COMB D. G., ROSEMAN S. Glucosamine metabolism. IV. Glucosamine-6-phosphate deaminase. J Biol Chem. 1958 Jun;232(2):807–827. [PubMed] [Google Scholar]
  3. DISCHE Z., BORENFREUND E. A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951 Oct;192(2):583–587. [PubMed] [Google Scholar]
  4. KLENK E., LAUENSTEIN K. Uber die zuckerhaltigen Lipoide des Erythrocytenstromas von Mensch und Rind. Hoppe Seylers Z Physiol Chem. 1953 Jan 30;291(5-6):249–258. [PubMed] [Google Scholar]
  5. LELOIR L. F., CARDINI C. E. Enzymes acting on glucosamine phosphate. Biochim Biophys Acta. 1956 Apr;20(1):33–42. doi: 10.1016/0006-3002(56)90259-1. [DOI] [PubMed] [Google Scholar]
  6. LEVVY G. A., MCALLAN A. The N-acetylation and estimation of hexosamines. Biochem J. 1959 Sep;73:127–132. doi: 10.1042/bj0730127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MORITA R. Y., NAKADA H. I., WOLFE J. B. Glucosamine degradation by Escherichia coli. I. Observations with resting cells and drycell preparations. Arch Biochem Biophys. 1956 Oct;64(2):480–488. doi: 10.1016/0003-9861(56)90290-9. [DOI] [PubMed] [Google Scholar]
  8. NAKADA H. I., WOLFE J. B. Glucosamine degradation by Escherichia coli. II. The isomeric conversion of glucosamine 6-PO4 to fructose 6-PO4 and ammonia. Arch Biochem Biophys. 1956 Oct;64(2):489–497. doi: 10.1016/0003-9861(56)90291-0. [DOI] [PubMed] [Google Scholar]
  9. PAYNE W. J., KIEBER R. J. The chromatographic determination of glucosamine with ninhydrin. Arch Biochem Biophys. 1954 Sep;52(1):1–4. doi: 10.1016/0003-9861(54)90082-x. [DOI] [PubMed] [Google Scholar]
  10. SAROJA K., VENKATARAMAN R., GIRI K. V. Transglucosidation in Penicillium chrysogenum Q-176; isolation and identification of the oligosaccharides. Biochem J. 1955 Jul;60(3):399–403. doi: 10.1042/bj0600399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SEEGMILLER J. E., HORECKER B. L. The synthesis of glucose-6-phosphate and 6-phosphogluconate. J Biol Chem. 1951 Sep;192(1):175–180. [PubMed] [Google Scholar]
  12. Schwimmer S., Bevenue A. Reagent for Differentiation of 1,4- and 1,6-Linked Glucosaccharides. Science. 1956 Mar 30;123(3196):543–544. doi: 10.1126/science.123.3196.543. [DOI] [PubMed] [Google Scholar]
  13. WICK A. N., DRURY D. R., NAKADA H. I., WOLFE J. B. Localization of the primary metabolic block produced by 2-deoxyglucose. J Biol Chem. 1957 Feb;224(2):963–969. [PubMed] [Google Scholar]
  14. WOLFE J. B., BRITTON B. B., NAKADA H. I. Glucosamine degradation by Escherichia coli. III. Isolation and studies of phosphoglucosaminisomerase. Arch Biochem Biophys. 1957 Feb;66(2):333–339. doi: 10.1016/s0003-9861(57)80008-3. [DOI] [PubMed] [Google Scholar]

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