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. 1964 May;87(5):1192–1197. doi: 10.1128/jb.87.5.1192-1197.1964

FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM

Robert R Banville a,1
PMCID: PMC277166  PMID: 5874541

Abstract

Banville, Robert R. (The Catholic University of America, Washington, D.C.). Factors affecting growth of Staphylococcus aureus L forms on semidefined medium. J. Bacteriol. 87:1192–1197. 1964.—A semidefined agar medium was found suitable for production and cultivation of the L form of Staphylococcus aureus. In semidefined liquid medium, growth of the L form took place in the form of a sediment containing large masses of cells, but heavy and diffuse growth occurred in the same medium with 0.05% agar. The optimal pH for L-colony formation on solid medium was 6.5. More L colonies developed on 0.75% agar than at higher agar concentrations. L colonies developed in greater numbers on pour plates than on streak plates, and in some cases more L colonies appeared under anaerobic incubation. L-colony formation appeared to be inhibited by sodium citrate. The vitamin requirements of the L forms studied were similar to those of the classical form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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