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. Author manuscript; available in PMC: 2009 Nov 3.
Published in final edited form as: Virology. 2007 May 2;365(1):157–165. doi: 10.1016/j.virol.2007.03.054

Figure 3. EBVgH is the binding partner of EBVgp42 and marmoset gH/gL is unable to bind to EBVgp42.

Figure 3

CHO-K1 were transiently transfected with EBV gp42, gB and different combinations of marmoset and EBV gH and gL, and cell biotinylated at 4°C (M=marmoset). Cells were lysed, immunoprecipitated with either an anti-HA Ab (HA.11)(A & B) or an anti-gp42 Ab (F-2-1) (C & D) and SDS-PAGE performed. Avidin-HRP was used to detect biotinylated proteins (A, C & D) and an anti-gp42 rabbit polyclonal Ab (PB1114) (B) to detect gp42 associating with gH/gL. Both EBVgL and MgL were labeled with an HA tag at their C-termini. EBVgL was omitted from transfection in lane 3 in both C and D. The proteins of interest are indicated with arrows. Positions of prestained protein markers (in kDa) are indicated.