Abstract
Reich, Claude V. (Johns Hopkins-Leonard Wood Memorial Leprosy Research Laboratory, Johns Hopkins University, Baltimore, Md.), and John H. Hanks. Use of Arthrobacter terregens for bioassay of mycobactin. J. Bacteriol. 87:1317–1320. 1964.—Arthrobacter terregens was used to assay mycobactin, a growth factor for Mycobacterium paratuberculosis. Within 7 days, A. terregens gave a linear photometric growth response to mycobactin in the range of 0.05 to 0.2 μg/ml. Preparations found to be active (or inactive) by this assay produced corresponding effects on the growth of M. paratuberculosis after 6 weeks to 4 months. Mycobactin was produced routinely from pellicles of M. phlei on a peptone-glycerol-beef heart infusion medium, and was extracted from both cells and medium by organic solvents. The mycobactin content per cell rose rapidly after the third day and attained a maximum at 4 to 6 days. The decline to less than one-half this value by the tenth day was associated with excretion into the medium. Production on synthetic media occurred after increasing the usual levels of asparagine. The demonstrated effects of crude mycobactin on the donor strain were (i) to catalyze the onset of growth and (ii) to reverse the effect of conditions which cause the formation of abnormal cells.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURNHAM B. F., NEILANDS J. B. Studies on the metabolic function of the ferrichrome compounds. J Biol Chem. 1961 Feb;236:554–559. [PubMed] [Google Scholar]
- BURTON M. O., SOWDEN F. J., LOCHHEAD A. G. Studies on the isolation and nature of the terregens factor. Can J Biochem Physiol. 1954 Jul;32(4):400–406. [PubMed] [Google Scholar]
- FRANCIS J., MACTURK H. M., MADINAVEITIA J., SNOW G. A. Mycobactin, a growth factor for Mycobacterium johnei. I. Isolation from Mycobacterium phlei. Biochem J. 1953 Nov;55(4):596–607. doi: 10.1042/bj0550596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOCHHEAD A. G. Soil bacteria and growth-promoting substances. Bacteriol Rev. 1958 Sep;22(3):145–153. doi: 10.1128/br.22.3.145-153.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NEILANDS J. B. Some aspects of microbial iron metabolism. Bacteriol Rev. 1957 Jun;21(2):101–111. doi: 10.1128/br.21.2.101-111.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH H. W. Modifications of Dubos's media for the cultivation of Mycobacterium johnei. J Pathol Bacteriol. 1953 Oct;66(2):375–381. doi: 10.1002/path.1700660206. [DOI] [PubMed] [Google Scholar]