Table 2.

Inter-day and intra-day assay precision, accuracy of the LC-MS method for the measurement of AEA in rat brain DVC tissue

Inter-day variation					Intra-day variation
Nominal concentration (ng mL-1)	Mean concentration found (ng mL-1)	Precision CV%	Mean accuracy (%)	%bias	Nominal concentration (ng mL-1)	Mean concentration found (ng mL-1)	Precision CV%	Mean accuracy (%)	%bias
AEA
1	1.1	12.2	106.3	6.3	1	1.0	14.8	102.8	2.8
25	24.5	15.2	97.9	-2.1	25	25.4	1.7	101.6	1.6
50	50.3	14.7	100.5	0.5	50	52.3	5.6	104.6	4.6
DEA
2	1.7	10.2	84.7	-15.3	2	1.8	9.4	91.8	-8.4
50	49.7	20.0	99.4	-0.6	50	54.1	2.5	108.1	8.1
100	100.1	16.6	100.1	0.1	100	106.3	6.0	106.3	6.3
NADA
1	1.0	3.0	103.9	3.9	1	1.0	13.6	100.4	0.4
25	24.5	12.5	98.1	-1.9	25	24.9	1.4	99.4	-0.6
50	50.2	10.6	100.4	0.4	50	50.6	2.2	101.1	1.1

Mean accuracy(%) = 100*(mean concentration/nominal concentration). % bias = 100*(mean concentration - nominal concentration)/nominal concentration. %CV = 100*(standard deviation/mean concentration)