Abstract
Karakawa, Walter W. (Connecticut State Department of Health, Hartford), Earle K. Borman, and Clarence R. McFarland. Typing of group A streptococci by immunofluorescence. I. Preparation and properties of type 1 fluorescein-labeled antibody. J. Bacteriol. 87:1377–1382. 1964.—Unadsorbed, fluorescein-labeled globulins derived from rabbits immunized with acid-extracted M-protein of type 1 streptococci (plus adjuvant) were found to have high fluorescent-antibody (FA) staining titers and to be considerably more type-specific than were similar preparations derived from whole-cell immunization. Appropriate adsorption rendered the anti-M reagent entirely type-specific without appreciable loss of titer; whole-cell reagent was appreciably weakened in FA titer by comparable adsorption. Type-specificity was confirmed by parallel bactericidal, long-chain, and precipitin studies. Removal of reactivity by adsorption with homologous M protein was complete, confirming that the FA reaction was truly a manifestation of an M anti-M protein system. The data indicate that the development of FA reagents specific for the streptococcal types is feasible.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARKULIS S. S., JONES M. F. Studies of streptococcal cell walls. I. Isolation, chemical composition, and preparation of M protein. J Bacteriol. 1957 Aug;74(2):207–216. doi: 10.1128/jb.74.2.207-216.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAUGHADAY W. H., LOWRY O. H., ROSEBROUGH N. J., FIELDS W. S. Determination of cerebrospinal fluid protein with the Folin phenol reagent. J Lab Clin Med. 1952 Apr;39(4):663–665. [PubMed] [Google Scholar]
- HAYASHI J. A., WALSH G. Studies of streptococcal cell walls. VI. Effects of adjuvants on the production of type-specific antibodies to cell walls and isolated M protein. J Bacteriol. 1961 Nov;82:736–742. doi: 10.1128/jb.82.5.736-742.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HIRSCH J. G., CHURCH A. B. Studies of phagocytosis of group A streptococci by polymorphonuclear leucocytes in vitro. J Exp Med. 1960 Mar 1;111:309–322. doi: 10.1084/jem.111.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANTOR F. S., COLE R. M. Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin. J Exp Med. 1960 Jul 1;112:77–96. doi: 10.1084/jem.112.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN M. H. Localization of streptococcal antigens in tissues. I. Histologic distribution and persistence of M protein, types 1, 5, 12, and 19 in the tissues of the mouse. J Exp Med. 1958 Mar 1;107(3):341–352. doi: 10.1084/jem.107.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LANCEFIELD R. C., PERLMANN G. E. Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus. J Exp Med. 1952 Jul;96(1):71–82. doi: 10.1084/jem.96.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
- MOODY M. D., ELLIS E. C., UPDYKE E. L. Staining bacterial smears with fluorescent antibody. IV. Grouping streptococci with fluorescent antibody. J Bacteriol. 1958 May;75(5):553–560. doi: 10.1128/jb.75.5.553-560.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOLLERMAN G. H., EKSTEDT R. Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction. J Exp Med. 1957 Sep 1;106(3):345–356. doi: 10.1084/jem.106.3.345. [DOI] [PMC free article] [PubMed] [Google Scholar]