Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Jul;88(1):48–54. doi: 10.1128/jb.88.1.48-54.1964

CULTIVATION OF LEPTOSPIRAE I.

Nutrition of Leptospira Canicola

O H V Stalheim a,1, J B Wilson a
PMCID: PMC277255  PMID: 14197904

Abstract

Stalheim, O. H. V. (University of Wisconsin, Madison), and J. B. Wilson. Cultivation of leptospirae. I. Nutrition of Leptospira canicola. J. Bacteriol. 88:48–54. 1964.—The nutrition of Leptospira canicola was investigated by use of synthetic media of suitable ionic strength. At an incubation temperature of 30 C, the minimal components were calcium, iron, magnesium, and ammonium ions, thiamine, and a fatty acid source; barium and strontium replaced calcium. Aspartic acid, glutamic acid, or methionine stimulated the rate and amount of growth; the best growth occurred in medium containing additional amino acids. Additions of cyanocobalamin or biotin permitted growth at 37 C. The stimulatory effects of added cyanocobalamin, biotin, pyridoxine, pantothenate, lipoic acid, or nicotinic acid were additive at 37 C, but not at 30 C. Fatty acids containing 14, 16, 17, or 18 carbon atoms supported growth; linoleic and linolenic acids were toxic. Glyceryl monooleate or trioleate, or Tween 40, 60, or 80 supported moderate to good growth; a mixture of monoolein and Tween 60, or Tweens 60 and 80 supported the best growth. Ten strains of L. canicola cultivated in a synthetic medium containing Tweens 60 and 80 attained cellular densities per ml of 107 to 4.0 × 107 organisms. L. canicola cells, resuspended in medium containing oleic-1-C14 acid, incorporated label primarily into cellular lipids; a lesser amount was located in the protein fraction, and only trace amounts were found in the nucleic acid fraction. The rate of incorporation was not affected by added sodium acetate. L. canicola was found to have fatty acid decarboxylase activity.

Full text

PDF
48

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FULTON J. D., SPOONER D. F. The metabolism of leptospira icterohaemorrhagiae in vitro. Exp Parasitol. 1956 Mar;5(2):154–177. doi: 10.1016/0014-4894(56)90012-1. [DOI] [PubMed] [Google Scholar]
  2. GERHARDT M. R., BALL M. G. Amino acid utilization by Leptospira canicola. J Bacteriol. 1959 Jan;77(1):17–22. doi: 10.1128/jb.77.1.17-22.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HAM R. G. Albumin replacement by fatty acids in clonal growth of mammalian cells. Science. 1963 May 17;140(3568):802–803. doi: 10.1126/science.140.3568.802. [DOI] [PubMed] [Google Scholar]
  4. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. II. FATTY ACID REQUIREMENTS. J Bacteriol. 1963 May;85:976–982. doi: 10.1128/jb.85.5.976-982.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. III. CALCIUM, MAGNESIUM, AND POTASSIUM REQUIREMENTS. J Bacteriol. 1963 May;85:983–985. doi: 10.1128/jb.85.5.983-985.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOHNSON R. C., GARY N. D. Nutrition of Leptospira pomona. 1. A chemically defined substitute for rabbit serum ultrafiltrate. J Bacteriol. 1962 Mar;83:668–672. doi: 10.1128/jb.83.3.668-672.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JOHNSON R. C., WILSON J. B. Nutrition of Leptospira pomona. J Bacteriol. 1960 Sep;80:406–411. doi: 10.1128/jb.80.3.406-411.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LARSON A. D., TREICK R. W., EDWARDS C. L., COX C. D. Growth studies and plate counting of leptospires. J Bacteriol. 1959 Mar;77(3):361–366. doi: 10.1128/jb.77.3.361-366.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MARKOVETZ A. J., LARSON A. D. Transamination in Leptospira biflexa. Proc Soc Exp Biol Med. 1959 Aug-Sep;101:638–640. doi: 10.3181/00379727-101-25044. [DOI] [PubMed] [Google Scholar]
  10. MENDELSOHN R. S., WATKIN D. M., HORBETT A. P., FAHEY J. L. Identification of the vitamin B12-binding protein in the serum of normals and of patients with chronic myelocytic leukemia. Blood. 1958 Aug;13(8):740–747. [PubMed] [Google Scholar]
  11. MOLLIN D. L., ROSS G. I. M. The vitamin B12 concentrations of serum and urine of normals and of patients with megaloblastic anaemias and other diseases. J Clin Pathol. 1952 May;5(2):129–139. doi: 10.1136/jcp.5.2.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SCHNEIDERMAN A., GREENE M. R., SCHIELER L., MCCLURE L. E., DUNN M. S. Nutrition of Leptospira canicola. III. Utilization of vitamins and amino acids. Proc Soc Exp Biol Med. 1953 Jan;82(1):53–56. doi: 10.3181/00379727-82-20021. [DOI] [PubMed] [Google Scholar]
  13. VOGEL H., HUTNER S. H. Growth of leptospira in defined media. J Gen Microbiol. 1961 Oct;26:223–230. doi: 10.1099/00221287-26-2-223. [DOI] [PubMed] [Google Scholar]
  14. WORATZ H. Wachstumsversuche mit Fettsäuren an Leptospira canicola. Zentralbl Bakteriol Orig. 1957 Aug;169(3-4):269–274. [PubMed] [Google Scholar]
  15. YANAGAWA R., HIRAMUNE T., FUJITA J. EFFECTS OF CARBON DIOXIDE ON THE COLONIAL GROWTH OF PATHOGENIC LEPTOSPIRAE. J Bacteriol. 1963 Apr;85:875–878. doi: 10.1128/jb.85.4.875-880.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. YANAGAWA R., WILSON J. B. Two types of Leptospirae distinguishable by their growth in boiled serum medium. J Infect Dis. 1962 Jan-Feb;110:70–74. doi: 10.1093/infdis/110.1.70. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES