Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Jul;88(1):179–186. doi: 10.1128/jb.88.1.179-186.1964

VALINE-ISOLEUCINE METABOLISM IN ACETOBACTER SUBOXYDANS AND THE INHIBITION OF GROWTH BY VALINE1

Suresh S Kerwar a,2, Vernon H Cheldelin a, L W Parks a
PMCID: PMC277275  PMID: 14197885

Abstract

Kerwar, Suresh S. (Oregon State University, Corvallis), Vernon, H. Cheldelin, and L. W. Parks. Valine-isoleucine metabolism in Acetobacter suboxydans and the inhibition of growth by valine. J. Bacteriol. 88:179–186. 1964.—Extracts of Acetobacter suboxydans can synthesize valine and isoleucine via acetolactate and acetohydroxybutyrate, respectively. The amounts of these amino acids synthesized from different intermediates were determined. The pathways appear to be identical to those described for yeast, Neurospora, and Escherichia coli. When exogenous valine was added to a synthetic growth medium inoculated with A. suboxydans, no growth of the culture was observed. The inhibitory effect of valine was reversed by the addition of isoleucine. The site and mechanism of valine inhibition were investigated. Threonine deaminase was inhibited by valine and isoleucine but not by leucine. Repression of the deaminase by isoleucine but not by valine was indicated. The data reported in this paper suggest that valine prevented growth of the organism through false feedback inhibition of threonine deaminase, thereby limiting isoleucine biosynthesis.

Full text

PDF
179

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANGEUX J. P. The feedback control mechanisms of biosynthetic L-threonine deaminase by L-isoleucine. Cold Spring Harb Symp Quant Biol. 1961;26:313–318. doi: 10.1101/sqb.1961.026.01.037. [DOI] [PubMed] [Google Scholar]
  2. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KING T. E., CHELDELIN V. H. Oxidations in Acetobacter suboxydans. Biochim Biophys Acta. 1954 May;14(1):108–116. doi: 10.1016/0006-3002(54)90137-7. [DOI] [PubMed] [Google Scholar]
  5. LEAVITT R. I., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. X. The enzymatic formation of acetohydroxybutyrate. J Biol Chem. 1961 Sep;236:2486–2491. [PubMed] [Google Scholar]
  6. LEAVITT R. I., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. XI. Valine inhibition of the growth of Escherichia coli strain K-12. J Bacteriol. 1962 Mar;83:624–630. doi: 10.1128/jb.83.3.624-630.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. MONOD J., CHANGEUX J. P., JACOB F. Allosteric proteins and cellular control systems. J Mol Biol. 1963 Apr;6:306–329. doi: 10.1016/s0022-2836(63)80091-1. [DOI] [PubMed] [Google Scholar]
  9. MOYED H. S. False feedback inhibition: inhibition of tryptophan biosynthesis by 5-methyltryptophan. J Biol Chem. 1960 Apr;235:1098–1102. [PubMed] [Google Scholar]
  10. MOYED H. S., UMBARGER H. E. Regulation of biosynthetic pathways. Physiol Rev. 1962 Jul;42:444–466. doi: 10.1152/physrev.1962.42.3.444. [DOI] [PubMed] [Google Scholar]
  11. Stokes J. L., Larsen A. Amino Acid Requirements of Acetobacter suboxydans. J Bacteriol. 1945 May;49(5):495–501. doi: 10.1128/jb.49.5.495-501.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. UMBARGER H. E., BROWN B. Threonine deamination in Escherichia coli. II. Evidence for two L-threonine deaminases. J Bacteriol. 1957 Jan;73(1):105–112. doi: 10.1128/jb.73.1.105-112.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES