Abstract
A. Keynan (Israel Institute of Biological Research, Ness Ziona, Israel), Z. Evenchik, H. O. Halvorson, and J. W. Hastings. Studies on the activation of bacterial endospores. J. Bacteriol. 88:313–318. 1964.—Heat activation of bacterial endospores was imitated by suspending spores in reducing agents (mercaptoethanol or thioglycolate) or in a pH less than 4.5. Urea (6 m) had no effect on spores. In addition to the well-known activation at 65 C for 45 min, spores were also activated by exposure to 34 C for 48 hr. The activation by heat and by reducing agents was reversible; the reverse reaction was temperature-dependent. No reversion occurred at −20 C, whereas at 28 C the spores reversed to their original dormant state within 72 hr.
It is suggested that the heat-activation phenomenon could be explained by assuming that heat or reducing agents change the tertiary structure of a protein responsible for the maintenance of the dormant state by reducing the disulfide linkages which stabilize the protein in a specific configuration. The partial denaturation of this protein is reversible by reoxidation of the reduced disulfide bonds.
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Selected References
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- CHURCH B. D., HALVORSON H. Intermediate metabolism of aerobic spores. I. Activation of glucose oxidation in spores of Bacillus cereus var terminalis. J Bacteriol. 1957 Apr;73(4):470–476. doi: 10.1128/jb.73.4.470-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans F. R., Curran H. R. The accelerating effect of sublethal heat on spore germination in mesophilic aerobic bacteria. J Bacteriol. 1943 Dec;46(6):513–523. doi: 10.1128/jb.46.6.513-523.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JANSSEN F. W., LUND A. J., ANDERSON L. E. Colorimetric assay for dipicolinic acid in bacterial spores. Science. 1958 Jan 3;127(3288):26–27. doi: 10.1126/science.127.3288.26. [DOI] [PubMed] [Google Scholar]
- KEYNAN A., MURRELL W. G., HALVORSON H. O. Germination properties of spores with low dipicolinic acid content. J Bacteriol. 1962 Feb;83:395–399. doi: 10.1128/jb.83.2.395-399.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINSON H. S., HYATT M. T. Some effects of heat and ionizing radiation on spores of Bacillus megaterium. J Bacteriol. 1960 Oct;80:441–451. doi: 10.1128/jb.80.4.441-451.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee W. H., Ordal Z. J. REVERSIBLE ACTIVATION FOR GERMINATION AND SUBSEQUENT CHANGES IN BACTERIAL SPORES. J Bacteriol. 1963 Jan;85(1):207–217. doi: 10.1128/jb.85.1.207-217.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'CONNOR R. J., HALVORSON H. O. L-Alanine dehydrogenase: a mechanism controlling the specificity of amino acid-induced germination of Bacillus cereus spores. J Bacteriol. 1961 Nov;82:706–713. doi: 10.1128/jb.82.5.706-713.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POWELL J. F., HUNTER J. R. Spore germination in the genus bacillus: the modification of germination requirements as a result of preheating. J Gen Microbiol. 1955 Aug;13(1):59–67. doi: 10.1099/00221287-13-1-59. [DOI] [PubMed] [Google Scholar]
- VINTER V. Spores of microorganisms. IX. Gradual development of the resistant structure of bacterial endospores. Folia Microbiol (Praha) 1962 Mar;7:115–120. doi: 10.1007/BF02927234. [DOI] [PubMed] [Google Scholar]