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. 1964 Aug;88(2):329–338. doi: 10.1128/jb.88.2.329-338.1964

EXTRACELLULAR POLYSACCHARIDES OF AZOTOBACTER VINELANDII1

Gary H Cohen a,2, Donald B Johnstone a
PMCID: PMC277304  PMID: 14203348

Abstract

Cohen, Gary H. (University of Vermont, Burlington), and Donald B. Johnstone. Extracellular polysaccharides of Azotobacter vinelandii. J. Bacteriol. 88:329–338. 1964.—Extracellular polysaccharides synthetized by Azotobacter vinelandii strains 155, 102, and 3A were shown to be carboxylic acid heteropolysaccharides of apparent high molecular weight. Cells were grown in a nitrogen-free, mineral broth medium with 2% sucrose. Extracellular slime was recovered by centrifugation and purified by repeated alcohol precipitation and Sevag deproteinization. Capsular polysaccharide was recovered from washed cells by mild alkaline digestion. Methods of isolation and purification appeared to provide polysaccharide showing no evidence of heterogeneity when examined by chemical and physical methods. Infrared analysis of purified slime from the three strains suggested fundamental structural similarities. Colorimetric, paper chromatographic, and enzymatic analyses on both intact and acid-hydrolyzed slime polysaccharide indicated that the polymers contained in common galacturonic acid, [α] d-glucose, and rhamnose at a ratio of approximately 43:2:1, as well as a hexuronic acid lactone, probably mannurono-lactone. However, as shown by chemical and infrared analysis, minor differences did exist; namely, slime from strain 155 and 102 contained o-acetyl groups, whereas slime from strain 3A contained none. A sialic acid-like component (1.5% of dry weight of the polysaccharide, calculated as N-acetyl neuraminic acid), was found only in the slime of strain 155. Capsular polysaccharide composition closely resembled that for slime. It is of interest that the major slime components were identical whether the energy source provided for the cells was sucrose, glucose, fructose, or ethanol.

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Selected References

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  1. BARKER S. A., FOSTER A. B., SIDDIQUI I. R., STACEY M. Structure of an acidic polysaccharide elaborated by Aerobacter aerogenes. Nature. 1958 Apr 5;181(4614):999–999. doi: 10.1038/181999a0. [DOI] [PubMed] [Google Scholar]
  2. BARRY G. T., ABBOTT V., TSAI T. Relationship of colominic acid (poly N-acetylneuraminic acid) to bacteria which contain neuraminic acid. J Gen Microbiol. 1962 Oct;29:335–352. doi: 10.1099/00221287-29-2-335. [DOI] [PubMed] [Google Scholar]
  3. BARRY G. T., HAMM J. D., GRAHAM M. G. EVALUATION OF COLORIMETRIC METHODS IN THE ESTIMATION OF SIALIC ACID IN BACTERIA. Nature. 1963 Nov 23;200:806–807. doi: 10.1038/200806a0. [DOI] [PubMed] [Google Scholar]
  4. BULEN W. A., LECOMTE J. R. Isolation and properties of a yellow-green fluorescent peptide from azotobacter medium. Biochem Biophys Res Commun. 1962 Dec 19;9:523–528. doi: 10.1016/0006-291x(62)90119-5. [DOI] [PubMed] [Google Scholar]
  5. COHEN G. H., JOHNSTONE D. B. Acid production by Azotobacter vinelandii. Nature. 1963 Apr 13;198:211–211. doi: 10.1038/198211a0. [DOI] [PubMed] [Google Scholar]
  6. DUBOIS M., GILLES K., HAMILTON J. K., REBERS P. A., SMITH F. A colorimetric method for the determination of sugars. Nature. 1951 Jul 28;168(4265):167–167. doi: 10.1038/168167a0. [DOI] [PubMed] [Google Scholar]
  7. Eklund C., Wyss O. ENZYME ASSOCIATED WITH BACTERIOPHAGE INFECTION. J Bacteriol. 1962 Dec;84(6):1209–1215. doi: 10.1128/jb.84.6.1209-1215.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEVINE S., STEVENSON H. J., KABLER P. W. Qualitative studies of pneumococcal polysaccharides by infrared spectrophotometry. Arch Biochem Biophys. 1953 Jul;45(1):65–73. doi: 10.1016/0003-9861(53)90405-6. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. RACUSEN D., JOHNSTONE D. B. Estimation of protein in cellular material. Nature. 1961 Jul 29;191:492–493. doi: 10.1038/191492a0. [DOI] [PubMed] [Google Scholar]
  11. SALTON M. R. Specific detection of glucose on paper chromatograms. Nature. 1960 Jun 18;186:966–967. doi: 10.1038/186966b0. [DOI] [PubMed] [Google Scholar]
  12. SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
  13. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  14. WILKINSON J. F. The extracellualr polysaccharides of bacteria. Bacteriol Rev. 1958 Mar;22(1):46–73. doi: 10.1128/br.22.1.46-73.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WYSS O., NEUMNN M. G., SOCOLOFSKY M. D. Development and germination of the Azotobacter cyst. J Biophys Biochem Cytol. 1961 Aug;10:555–565. doi: 10.1083/jcb.10.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]

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