Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Aug;88(2):389–394. doi: 10.1128/jb.88.2.389-394.1964

BIOTIN DEFICIENCY AND THE FATTY ACIDS OF CERTAIN BIOTIN-REQUIRING BACTERIA1

Jo A Croom 1,2, J J McNeill 1,2, S B Tove 1,2
PMCID: PMC277312  PMID: 14203355

Abstract

Croom, Jo A. (North Carolina State of the University of North Carolina, Raleigh), J. J. McNeill, and S. B. Tove. Biotin deficiency and the fatty acids of certain biotin-requiring bacteria. J. Bacteriol. 88:389–394. 1964.—The major fatty acids of Lactobacillus plantarum were identified as myristic, palmitic, palmitoleic, stearic, cis-vaccenic, and lactobacillic. Small amounts of a C14-monoenoic acid were also found. The major acids of a biotin-requiring mutant of Escherichia coli were lauric, myristic, palmitic, palmitoleic, methylene-hexadecanoic, octadecenoic, and methylene-octadecanoic. The C16-monoenoic acid fraction of E. coli also contained small amounts of 7,8-hexadecenoate. The C18-monoenoic acid fraction contained 9,10-octadecenoic acid (oleic), with small amounts of 7,8-octadecenoic. Two other components, which had properties similar to tridecanoic and pentadecanoic acids, were also found. Biotin deficiency in L. plantarum decreased the relative amounts of cis-vaccenic and lactobacillic acids, and increased palmitic acid. In E. coli there was no change in the proportion of palmitic acid, whereas there were increases in monoenoic acids and a decreased level of methylene-hexadecanoic acid. In L. plantarum, palmitic and lactobacillic acids increased with age, and cis-vaccenic decreased sharply. In biotin deficiency, there was a decrease of about 20% in total lipid of L. plantarum.

Full text

PDF
389

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOCH K., BARONOWSKY P., GOLDFINE H., LENNARZ W. J., LIGHT R., NORRIS A. T., SCHEUERBRANDT G. Biosynthesis and metabolism of unsaturated fatty acids. Fed Proc. 1961 Dec;20:921–927. [PubMed] [Google Scholar]
  2. FERGUSON R. B., LICHSTEIN H. C. Biotin requirements of a mutant strain of Escherichia coli. Proc Soc Exp Biol Med. 1957 Aug-Sep;95(4):766–769. [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. GOLDFINE H., BLOCH K. On the origin of unsaturated fatty acids in clostridia. J Biol Chem. 1961 Oct;236:2596–2601. [PubMed] [Google Scholar]
  5. HOFMANN K., HENIS D. B., PANOS C. Fatty acid interconversions in lactobacilli. J Biol Chem. 1957 Sep;228(1):349–355. [PubMed] [Google Scholar]
  6. HOFMANN K., LUCAS R. A., SAX S. M. The chemical nature of the fatty acids of Lactobacillus arabinosus. J Biol Chem. 1952 Apr;195(2):473–485. [PubMed] [Google Scholar]
  7. JAMES A. T. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. Methods Biochem Anal. 1960;8:1–59. doi: 10.1002/9780470110249.ch1. [DOI] [PubMed] [Google Scholar]
  8. KANESHIRO T., MARR A. G. cis-9,10-Methylene hexadecanoic acid from the phospholipids of Escherichia coli. J Biol Chem. 1961 Oct;236:2615–2619. [PubMed] [Google Scholar]
  9. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SCHEURBRANDT G., BLOCH K. Unsaturated fatty acids in microorganisms. J Biol Chem. 1962 Jul;237:2064–2068. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES