Abstract
Jenkins, E. M. (Tuskegee Institute, Tuskegee, Ala.), A. N. Njoku-Obi, and E. W. Adams. Purification of the soluble hemolysins of Listeria monocytogenes. J. Bacteriol. 88:418–424. 1964.—A method is described for obtaining relatively purified hemolysin preparations from both virulent and avirulent strains of Listeria monocytogenes. These hemolysins are protein in nature as shown by heat lability, nondialyzable properties, precipitation with trichloroacetic acid, and electrophoretic mobility. The hemolysins are antigenic in rabbits as shown by serum neutralization tests. The potency of the purified hemolysin was markedly increased by cysteine, sodium hydrosulfite, and a number of reducing agents. Many of the actions of the purified hemolysin seemed to parallel that of streptolysin O, and certain of these activities could be explained by the “thioldisulfide hypothesis.”
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GIRARD K. F., SBARRA A. J., BARDAWIL W. A. Serology of Listeria monocytogenes. I. Characteristics of the soluble hemolysin. J Bacteriol. 1963 Feb;85:349–355. doi: 10.1128/jb.85.2.349-355.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herbert D., Todd E. W. Purification and properties of a haemolysin produced by group A haemolytic streptococci (streptolysin O). Biochem J. 1941 Nov;35(10-11):1124–1139. doi: 10.1042/bj0351124. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macfarlane M. G., Knight B. C. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins. Biochem J. 1941 Sep;35(8-9):884–902. doi: 10.1042/bj0350884. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NJOKU-OBI A. N., JENKINS E. M., NJOKU-OBI J. C., ADAMS J., COVINGTON V. PRODUCTION AND NATURE OF LISTERIA MONOCYTOGENES HEMOLYSINS. J Bacteriol. 1963 Jul;86:1–8. doi: 10.1128/jb.86.1.1-8.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]