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. 1964 Sep;88(3):553–558. doi: 10.1128/jb.88.3.553-558.1964

EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS II.

Carbon Dioxide Fixation Into Oxalacetate by Cell-Free Extracts

C L Baugh 1, J W Lanham 1, M J Surgalla 1
PMCID: PMC277346  PMID: 14208487

Abstract

Baugh, C. L. (Fort Detrick, Frederick, Md.), J. W. Lanham, and M. J. Surgalla. Effects of bicarbonate on growth of Pasteurella pestis. II. Carbon dioxide fixation into oxalacetate by cell-free extracts. J. Bacteriol. 88:553–558. 1964.—Enzyme preparations from Pasteurella pestis will carboxylate phosphoenolpyruvate to form oxalacetate by two distinct reactions. The reactions are similar to those catalyzed by the enzymes, phosphoenolpyruvic carboxylase and phosphoenolpyruvate carboxykinase. No significant differences in enzyme characteristics or enzyme content were found when virulent cells were compared with avirulent under the conditions of our experiments. The carboxykinase of P. pestis differs from that of animal origin, because it is dependent upon adenine derivatives rather than inosine or guanosine nucleotides. The latter two nucleotides can act indirectly by way of adenosine nucleotides, because nucleoside diphosphokinase and myokinase are present in the extract.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANDURSKI R. S., GREINER C. M. The enzymatic synthesis of oxalacetate from phosphoryl-enolpyruvate and carbon dioxide. J Biol Chem. 1953 Oct;204(2):781–786. [PubMed] [Google Scholar]
  2. BAUGH C. L., CLAUS G. W., WERKMAN C. H. Heterotrophic fixation of carbon dioxide by extracts of Nocardia corallina. Arch Biochem Biophys. 1960 Feb;86:255–259. doi: 10.1016/0003-9861(60)90414-8. [DOI] [PubMed] [Google Scholar]
  3. CANNATA J., STOPPANI A. O. Adenosine polyphosphate requirement of baker's yeast phosphopyruvate carboxylase. Biochim Biophys Acta. 1959 Mar;32(1):284–285. doi: 10.1016/0006-3002(59)90591-8. [DOI] [PubMed] [Google Scholar]
  4. DELWICHE E. A., FUKUI G. M., ANDREWS A. W., SURGALLA M. J. Environmental conditions affecting the population dynamics and the retention of virulence of Pasteurella pestis: the role of carbon dioxide. J Bacteriol. 1959 Mar;77(3):355–360. doi: 10.1128/jb.77.3.355-360.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FUKUI G. M., OGG J. E., WESSMAN G. E., SURGALLA M. J. Studies on the relation of cultural conditions and virulence of Pasteurella pestis. J Bacteriol. 1957 Dec;74(6):714–717. doi: 10.1128/jb.74.6.714-717.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GRIFFIN P. J., RACKER E. The carbon dioxide requirement of Neisseria gonorrhoeae. J Bacteriol. 1956 Jun;71(6):717–721. doi: 10.1128/jb.71.6.717-721.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HIGUCHI K., CARLIN C. E. Studies on the nutrition and physiology of Pasteurella pestis. II. A defined medium for the growth of Pasteurella pestis. J Bacteriol. 1958 Apr;75(4):409–413. doi: 10.1128/jb.75.4.409-413.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HIGUCHI K., KUPFERBERG L. L., SMITH J. L. Studies on the nutrition and physiology of Pasteurella pestis. III. Effects of calcium ions on the growth of virulent and avirulent strains of Pasteurella pestis. J Bacteriol. 1959 Mar;77(3):317–321. doi: 10.1128/jb.77.3.317-321.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. OGG J. E., FRIEDMAN S. B., ANDREWS A. W., SURGALLA M. J. Factors influencing the loss of virulence in Pasteurella pestis. J Bacteriol. 1958 Aug;76(2):185–191. doi: 10.1128/jb.76.2.185-191.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SURGALLA M. J., ANDREWS A. W., BAUGH C. L. EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS. I. DIFFERENTIAL RESPONSE OF VIRULENT AND AVIRULENT CELLS. J Bacteriol. 1964 Aug;88:269–272. doi: 10.1128/jb.88.2.269-272.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SUZUKI I., WERKMAN C. H. Chemoautotrophic carbon dioxide fixation by extracts of Thiobacillus thiooxidans. I. Formation of oxalacetic acid. Arch Biochem Biophys. 1958 Jul;76(1):103–111. doi: 10.1016/0003-9861(58)90124-3. [DOI] [PubMed] [Google Scholar]
  12. SUZUKI I., WERKMAN C. H. Phosphoenolpyruvate carboxylase in extracts of Thiobacillus thiooxidans, a chemoautotrophic bacterium. Arch Biochem Biophys. 1957 Dec;72(2):514–515. doi: 10.1016/0003-9861(57)90227-8. [DOI] [PubMed] [Google Scholar]
  13. TCHEN T. T., VENNESLAND B. Enzymatic carbon dioxide fixation into oxal-acetate in wheat germ. J Biol Chem. 1955 Apr;213(2):533–546. [PubMed] [Google Scholar]
  14. UTTER M. F., KURAHASHI K. Purification of oxalacetic carboxylase from chicken liver. J Biol Chem. 1954 Apr;207(2):787–802. [PubMed] [Google Scholar]

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