Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Sep;88(3):595–601. doi: 10.1128/jb.88.3.595-601.1964

INTRASPECIFIC AND INTERSPECIFIC TRANSFORMATION IN STREPTOCOCCI1

Dennis Perry a, Hutton D Slade a
PMCID: PMC277353  PMID: 14208494

Abstract

Perry, Dennis (Northwestern University Medical School, Chicago, Ill.), and Hutton D. Slade. Intraspecific and interspecific transformation in streptococci. J. Bacteriol. 88:595–601. 1964.—Interspecific transformation reactions, employing streptomycin resistance as a marker, were performed with eight strains of streptococci belonging to three serological groups (F, H, and O) and one ungroupable (UG) strain. Generally, autotransformation (within the same strain) was most efficient. Homotransformation (between different strains of the same serological group), however, was sometimes as efficient or slightly better. Heterotransformation (between different serological groups) yielded the least number of transformants or none at all. The rate of transformation of different strains varied from 2.0 × 10−7 to 7.6 × 10−3. Group H streptococci exhibited the highest rate of autotransformation, followed by groups F and O, and strain UG. The results of heterotransformation reactions revealed that a genetic relationship exists between various strains of different serological groups. No transformation, however, occurred between F and O strains, indicating a lack of genetic homology between these serological groups of streptococci. Deoxyribonucleic acid (DNA), isolated by physical and enzymatic methods from a group O and a UG strain, failed to induce transformation. DNA from these strains, however, significantly inhibited the transforming ability of other DNA preparations. Heat and mechanical shear resulted in a marked decrease in this inhibitory property.

Full text

PDF
595

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER H. E., LEIDY G., HAHN E. Studies on the nature of hemophilus influenzae cells susceptible to heritable changes by desoxyribonucleic acids. J Exp Med. 1954 Jun 1;99(6):505–533. doi: 10.1084/jem.99.6.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRACCO R. M., KRAUSS M. R., ROE A. S., MACLEOD C. M. Transformation reactions between Pneumococcus and three strains of Streptococci. J Exp Med. 1957 Aug 1;106(2):247–259. doi: 10.1084/jem.106.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CATLIN B. W., CUNNINGHAM L. S. Transforming activities and base contents of deoxyribonucleate preparations from various Neisseriae. J Gen Microbiol. 1961 Oct;26:303–312. doi: 10.1099/00221287-26-2-303. [DOI] [PubMed] [Google Scholar]
  4. GREEN D. M. A host-specific variation affecting relative frequency of transformation of two markers in pneumococcus. Exp Cell Res. 1959 Nov;18:466–480. doi: 10.1016/0014-4827(59)90312-x. [DOI] [PubMed] [Google Scholar]
  5. Hotchkiss R. D. CYCLICAL BEHAVIOR IN PNEUMOCOCCAL GROWTH AND TRANSFORMABILITY OCCASIONED BY ENVIRONMENTAL CHANGES. Proc Natl Acad Sci U S A. 1954 Feb;40(2):49–55. doi: 10.1073/pnas.40.2.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KRAUSS M. R., MACLEOD C. M. INTRASPECIES AND INTERSPECIES TRANSFORMATION REACTIONS IN PNEUMOCOCCUS AND STREPTOCOCCUS. J Gen Physiol. 1963 Jul;46:1141–1150. doi: 10.1085/jgp.46.6.1141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MARMUR J., FALKOW S., MANDEL M. NEW APPROACHES TO BACTERIAL TAXONOMY. Annu Rev Microbiol. 1963;17:329–372. doi: 10.1146/annurev.mi.17.100163.001553. [DOI] [PubMed] [Google Scholar]
  8. Marmur J., Seaman E., Levine J. INTERSPECIFIC TRANSFORMATION IN BACILLUS. J Bacteriol. 1963 Feb;85(2):461–467. doi: 10.1128/jb.85.2.461-467.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PAKULA R., HULANICKA E., WALCZAK W. Transformation reactions between different species of streptococci and between streptococci, pneumococci and staphylococci. Schweiz Z Pathol Bakteriol. 1959;22(2):202–214. doi: 10.1159/000160592. [DOI] [PubMed] [Google Scholar]
  10. PAKULA R. Interspecific transformation as a means of determining genetic relationships in streptococci. Acta Microbiol Pol. 1961;10:249–254. [PubMed] [Google Scholar]
  11. PERRY D., SLADE H. D. OPTIMAL CONDITIONS FOR THE TRANSFORMATION OF STREPTOCOCCI. J Bacteriol. 1963 Mar;85:636–642. doi: 10.1128/jb.85.3.636-642.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PERRY D., SLADE H. D. Transformation of streptococci to streptomycin resistance. J Bacteriol. 1962 Mar;83:443–449. doi: 10.1128/jb.83.3.443-449.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAVIN A. W., DESA J. H. GENETIC LINKAGE OF MUTATIONAL SITES AFFECTING SIMILAR CHARACTERS IN PNEUMOCOCCUS AND STREPTOCOCCUS. J Bacteriol. 1964 Jan;87:86–96. doi: 10.1128/jb.87.1.86-96.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SAGERS R. D. An efficient, easily constructed cell homogenizing press. Appl Microbiol. 1962 Jan;10:37–39. doi: 10.1128/am.10.1.37-39.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SCHAEFFER P., EDGAR R. S., ROLFE R. [On the inhibition of bacterial transformation by desoxyribonucleates having various structures]. C R Seances Soc Biol Fil. 1960;154:1978–1983. [PubMed] [Google Scholar]
  16. SCHAEFFER P. Interspecific reactions in bacterial transformation. Symp Soc Exp Biol. 1958;12:60–74. [PubMed] [Google Scholar]
  17. SCHAEFFER P. La pénétration de l'acide nucleique dans les bactéries réceptrices au cours des transformations interspécifiques. C R Hebd Seances Acad Sci. 1957 Jul 17;245(3):375–377. [PubMed] [Google Scholar]
  18. YOUNG F. E., SPIZIZEN J. Physiological and genetic factors affecting transformation of Bacillus subtilis. J Bacteriol. 1961 May;81:823–829. doi: 10.1128/jb.81.5.823-829.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES