Abstract
Hancock, R. (Harvard Medical School, Boston, Mass.). Early effects of streptomycin on Bacillus megaterium. J. Bacteriol. 88:633–639. 1964.—The sequence of some early effects of streptomycin on Bacillus megaterium was investigated. An increase in the rate of efflux of cellular K ions preceded loss of viability, but efflux of Mg ions remained normal. Loss of viability was closely coincident with reduction in rate of protein synthesis. Nucleic acid synthesis continued after protein synthesis ceased in low-phosphate medium, but ceased simultaneously with protein synthesis in normal minimal medium. Later, after viability had begun to decrease, the respiration rate began to fall and the cellular adenosine triphosphate (ATP) content decreased slowly. No leakage of ultraviolet-absorbing materials into the medium occurred, although uracil was slowly released from nucleic acid after killing had begun. It is concluded that in this organism, as previously observed with Escherichia coli, the actions of streptomycin which led to an increased rate of efflux of K and specific inhibition of protein synthesis are the only ones early enough to be of significance in its bactericidal action, the other effects being later secondary changes.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANAND N., DAVIS B. D., ARMITAGE A. K. Uptake of streptomycin by Escherichia coli. Nature. 1960 Jan 2;185:23–24. doi: 10.1038/185023a0. [DOI] [PubMed] [Google Scholar]
- ANAND N., DAVIS B. D. Damage by streptomycin to the cell membrane of Escherichia coli. Nature. 1960 Jan 2;185:22–23. doi: 10.1038/185022a0. [DOI] [PubMed] [Google Scholar]
- BARDI U., BORETTI G., DI MARCO A. Inhibition of oxidative phosphorylation by aminosidine and other antibiotics, in rat liver mitochondria. Biochem Pharmacol. 1961 Aug;7:165–168. doi: 10.1016/0006-2952(61)90082-x. [DOI] [PubMed] [Google Scholar]
- BARKULIS I. L. Inhibition of the anaerobic pyruvate metabolism of Escherichia coli by dihydrostreptomycin. J Bacteriol. 1953 Mar;65(3):337–343. doi: 10.1128/jb.65.3.337-343.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRAGG P. D., POLGLASE W. J. INHIBITION OF OXIDATIVE PHOSPHORYLATION IN ESCHERICHIA COLI BY DIHYDROSTREPTOMYCIN. J Bacteriol. 1963 Dec;86:1236–1240. doi: 10.1128/jb.86.6.1236-1240.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIES J., GILBERT W., GORINI L. STREPTOMYCIN, SUPPRESSION, AND THE CODE. Proc Natl Acad Sci U S A. 1964 May;51:883–890. doi: 10.1073/pnas.51.5.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUBIN D. T., DAVIS B. D. The effect of streptomycin on potassium flux in Escherichia coli. Biochim Biophys Acta. 1961 Sep 16;52:400–402. doi: 10.1016/0006-3002(61)90697-7. [DOI] [PubMed] [Google Scholar]
- DUBIN D. T., DAVIS B. D. The streptomycin-triggered depoly-merization of ribonucleic acid in Escherichia coli. Biochim Biophys Acta. 1962 May 14;55:793–795. doi: 10.1016/0006-3002(62)90863-6. [DOI] [PubMed] [Google Scholar]
- DUBIN D. T., HANCOCK R., DAVIS B. D. THE SEQUENCE OF SOME EFFECTS OF STREPTOMYCIN IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Aug 13;74:476–489. doi: 10.1016/0006-3002(63)91390-8. [DOI] [PubMed] [Google Scholar]
- FLAKS J. G., COX E. C., WHITE J. R. Inhibition of polypeptide synthesis by streptomycin. Biochem Biophys Res Commun. 1962 May 11;7:385–389. doi: 10.1016/0006-291x(62)90320-0. [DOI] [PubMed] [Google Scholar]
- HANCOCK R. Reduced oxidative activities in Escherichia coli and Bacillus megaterium in relation to other changes during inhibition of growth by streptomycin. J Gen Microbiol. 1961 Jul;25:429–440. doi: 10.1099/00221287-25-3-429. [DOI] [PubMed] [Google Scholar]
- HANCOCK R. The bactericidal action of streptomycin on Staphylococcus aureus and some accompanying biochemical changes. J Gen Microbiol. 1960 Aug;23:179–196. doi: 10.1099/00221287-23-1-179. [DOI] [PubMed] [Google Scholar]
- HANCOCK R. Uptake of 14C-streptomycin by Bacillus megaterium. J Gen Microbiol. 1962 Jul;28:503–516. doi: 10.1099/00221287-28-3-503. [DOI] [PubMed] [Google Scholar]
- Henry R. J., Housewright R. D., Berkman S. STUDIES ON STREPTOMYCIN VI. : The Effect of Streptomycin on the Metabolism of Multiplying Bacteria. J Bacteriol. 1949 Apr;57(4):447–451. [PMC free article] [PubMed] [Google Scholar]
- JACKSON F. L. Streptomycin and bacterial respiration. Nature. 1958 Jan 24;181(4604):281–282. doi: 10.1038/181281b0. [DOI] [PubMed] [Google Scholar]
- ROSANO C. L., PEABODY R. A., HURWITZ C. Studies on the mechanism of action of action of streptomycin. Effect of streptomycin on the excretion of nucleotides by Escherichia coli. Biochim Biophys Acta. 1960 Jan 15;37:380–382. doi: 10.1016/0006-3002(60)90261-4. [DOI] [PubMed] [Google Scholar]
- SPEYER J. F., LENGYEL P., BASILIO C. Ribosomal localization of streptomycin sensitivity. Proc Natl Acad Sci U S A. 1962 Apr 15;48:684–686. doi: 10.1073/pnas.48.4.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SPOTTS C. R., STANIER R. Y. Mechanism of streptomycin action on bacteria: a unitary hypothesis. Nature. 1961 Nov 18;192:633–637. doi: 10.1038/192633a0. [DOI] [PubMed] [Google Scholar]
- TZAGOLOFF H., UMBREIT W. W. Influence of streptomycin on nucleotide excretion in Escherichia coli. J Bacteriol. 1963 Jan;85:49–52. doi: 10.1128/jb.85.1.49-52.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WADE H. E., ROBINSON H. K. ABSENCE OF RIBONUCLEASE FROM THE RIBOSOMES OF PSEUDOMONAS FLUORESCENS. Nature. 1963 Nov 16;200:661–663. doi: 10.1038/200661a0. [DOI] [PubMed] [Google Scholar]