Abstract
Dudman, W. F. (Commonwealth Scientific and Industrial Research Organization, Canberra, Australia). Growth and extracellular polysaccharide production by Rhizobium meliloti in defined medium. J. Bacteriol. 88:640–645. 1964.—A defined medium was developed in which Rhizobium meliloti grew well in shaken flasks and produced large yields of extracellular polysaccharide. The medium contains 1% mannitol plus 1% sodium succinate as the carbon source. 0.2% sodium glutamate as the nitrogen source, mineral salts, thiamine, and biotin. Sucrose, glucose, and fructose were inferior to mannitol when compared as carbon sources. The presence of succinate in the medium enhanced polysaccharide production and also stimulated the appearance of an extracellular soluble pigment. In this medium, both growth and polysaccharide production were favored by low aeration.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DAVIS R. J., CLAPP C. E. Preparation of purified polysaccharides from Rhizobium. Appl Microbiol. 1961 Nov;9:519–524. doi: 10.1128/am.9.6.519-524.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUDMAN W. F. Cellulose production by Acetobacter actigenum in defined medium. J Gen Microbiol. 1959 Oct;21:327–337. doi: 10.1099/00221287-21-2-327. [DOI] [PubMed] [Google Scholar]
- DUDMAN W. F. IMMUNE DIFFUSION ANALYSIS OF THE EXTRACELLULAR SOLUBLE ANTIGENS OF TWO STRAINS OF RHIZOBIUM MELILOTI. J Bacteriol. 1964 Sep;88:782–794. doi: 10.1128/jb.88.3.782-794.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUGUID J. P., WILKINSON J. F. The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. J Gen Microbiol. 1953 Oct;9(2):174–189. doi: 10.1099/00221287-9-2-174. [DOI] [PubMed] [Google Scholar]
- SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
- VINCENT J. M. Influence of calcium and magnesium on the growth of rhizobium. J Gen Microbiol. 1962 Sep;28:653–663. doi: 10.1099/00221287-28-4-653. [DOI] [PubMed] [Google Scholar]
- WILKINSON J. F., DUGUID J. P., EDMUNDS P. N. The distribution of polysaccharide production in Aerobacter and Escherichia strains and its relation to antigenic character; with a note on the influence of potassium deficiency upon production of polysaccharide by Aerobacter aerogenes. J Gen Microbiol. 1954 Aug;11(1):59–72. doi: 10.1099/00221287-11-1-59. [DOI] [PubMed] [Google Scholar]