Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Nov;88(5):1288–1295. doi: 10.1128/jb.88.5.1288-1295.1964

PURIFICATION AND PROPERTIES OF N-ACETYL-d-GLUCOSAMINE KINASE FROM STREPTOCOCCUS PYOGENES

L D Zeleznick a,1, H Hankin a, J J Boltralik a, H Heymann a, S S Barkulis a
PMCID: PMC277406  PMID: 14234783

Abstract

Zeleznick, L. D. (CIBA Pharmaceutical Co., Summit, N.J.), H. Hankin, J. J. Boltralik, H. Heymann, and S. S. Barkulis. Purification and properties of N-acetyl-d-glucosamine kinase from Streptococcus pyogenes. J. Bacteriol. 88:1288–1295. 1964.—A kinase from Streptococcus pyogenes which catalyzes adenosine triphosphate-dependent phosphorylation of d-glucose and N-acetyl-d-glucosamine has been purified 1,500-fold. The ratio of the enzymatic activity on both substrates remained constant throughout the fractionation. Similarity in heat stability, p-hydroxymercuribenzoate inhibition, protection by either carbohydrate, and lack of repression of enzymatic activity when the bacteria were grown exclusively on one of the two substrates supports the hypothesis that kinase activity is associated with one enzyme.

Full text

PDF
1288

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARKULIS S. S., JONES M. F. Studies of streptococcal cell walls. I. Isolation, chemical composition, and preparation of M protein. J Bacteriol. 1957 Aug;74(2):207–216. doi: 10.1128/jb.74.2.207-216.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROWN D. H. The phosphorylation of D (+) glucosamine by crystalline yeast hexokinase. Biochim Biophys Acta. 1951 Nov;7(4):487–493. doi: 10.1016/0006-3002(51)90063-7. [DOI] [PubMed] [Google Scholar]
  3. DAVIDSON E. A., BLUMENTHAL H. J., ROSEMAN S. Glucosamine metabolism. II. Studies on glucosamine 6-phosphate N-acetylase. J Biol Chem. 1957 May;226(1):125–133. [PubMed] [Google Scholar]
  4. DISTLER J. J., MERRICK J. M., ROSEMAN S. Glucosamine metabolism. III. Preparation and N-acetylation of crystalline D-glucosamine- and D-galactosamine-6-phosphoric acids. J Biol Chem. 1958 Jan;230(1):497–509. [PubMed] [Google Scholar]
  5. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FAULKNER P., QUASTEL J. H. Anaerobic deamination of D-glucosamine by bacterial and brain extracts. Nature. 1956 Jun 30;177(4522):1216–1218. doi: 10.1038/1771216a0. [DOI] [PubMed] [Google Scholar]
  7. HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
  8. JEANLOZ R. W., FORCHIELLI E. Studies on hyaluronic acid and related substances. II. Periodate oxidation of glucosamine and derivatives. J Biol Chem. 1951 Jan;188(1):361–369. [PubMed] [Google Scholar]
  9. KORNBERG A., PRICER W. E., Jr Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside. J Biol Chem. 1951 Dec;193(2):481–495. [PubMed] [Google Scholar]
  10. LELOIR L. F., CARDINI C. E., OLAVARRIA J. M. Phosphorylation of acetylhexosamines. Arch Biochem Biophys. 1958 Mar;74(1):84–91. doi: 10.1016/0003-9861(58)90201-7. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. PLANAS J., DE CASTRO S. [The transport of blood iron in some mammals]. Rev Esp Fisiol. 1960 Jun;16:121–128. [PubMed] [Google Scholar]
  13. REISSIG J. L., STORMINGER J. L., LELOIR L. F. A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem. 1955 Dec;217(2):959–966. [PubMed] [Google Scholar]
  14. ROSEMAN S., LUDOWIEG J., MOSES F. E., DORFMAN A. The biosynthesis of hyaluronic acid by group A Streptococcus. II. Origin of the glucuronic acid. J Biol Chem. 1954 Feb;206(2):665–669. [PubMed] [Google Scholar]
  15. SCHMIDT W. C. Group A streptococcus polysaccharide: studies on its preparation, chemical composition, and cellular localization after intravenous injection into mice. J Exp Med. 1952 Jan;95(1):105–118. doi: 10.1084/jem.95.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHONBAUM E. Adrenocortical function in rats exposed to low environmental temperatures. Fed Proc. 1960 Dec;19(Suppl 5):85–88. [PubMed] [Google Scholar]
  17. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES