Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Nov;88(5):1356–1363. doi: 10.1128/jb.88.5.1356-1363.1964

LIPOLYTIC ACTIVITIES OF STAPHYLOCOCCUS AUREUS

I. Nature of the Enzyme Producing Free Fatty Acids From Plasma Lipids

William M O'Leary 1,2, Julia T Weld 1,2
PMCID: PMC277416  PMID: 14234793

Abstract

O'Leary, William M. (Cornell University Medical College, New York, N.Y.), and Julia T. Weld. Lipolytic activities of Staphylococcus aureus. I. Nature of the enzyme producing free fatty acids from plasma lipids. J. Bacteriol. 88:1356–1363. 1964.—The enzyme of Staphylococcus aureus, Guy strain, which is responsible for producing free fatty acids in human plasmas was purified approximately 225-fold from extracts of cultures in which the organism was grown. Purification procedures included ammonium sulfate precipitation, ethanol precipitation, gel filtration, and diethylaminoethyl-cellulose chromatography. Stability characteristics, pH and temperature optima, and substrate requirements were established. The principal plasma substrate is plasma lipoprotein, and the major reaction product is free octadecenoic acid. The ability of this compound to uncouple oxidative phosphorylation may be involved in the pathological effects of S. aureus at the molecular level.

Full text

PDF
1356

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOBEL H., BERMAN D. T., SIMON J. Purification of staphylococcal coagulase. J Bacteriol. 1960 Jun;79:807–815. doi: 10.1128/jb.79.6.807-815.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BORST P., LOOS J. A., CHRIST E. J., SLATER E. C. Uncoupling activity of long-chain fatty acids. Biochim Biophys Acta. 1962 Aug 27;62:509–518. doi: 10.1016/0006-3002(62)90232-9. [DOI] [PubMed] [Google Scholar]
  3. DAVIES M. E. A study of diffusible lipase produced by staphylococci and of its immunological activity. J Gen Microbiol. 1954 Aug;11(1):37–44. doi: 10.1099/00221287-11-1-37. [DOI] [PubMed] [Google Scholar]
  4. DESNUELLE P., CONSTANTIN M. J., BALDY J. Technique potentiométrique pour la measure de l'activité de la lipase pancréatique. Bull Soc Chim Biol (Paris) 1955;37(2-3):285–290. [PubMed] [Google Scholar]
  5. FUSILLO M. H., WEISS D. L. EFFECT OF HUMAN SERUM ON FATALITY OF STAPHYLOCOCCAL INFECTION IN MICE. J Bacteriol. 1963 Aug;86:212–215. doi: 10.1128/jb.86.2.212-215.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GILLESPIE W. A., ALDER V. G. Production of opacity in egg-yolk media by coagulase-positive staphylococci. J Pathol Bacteriol. 1952 Jan;64(1):187–200. doi: 10.1002/path.1700640119. [DOI] [PubMed] [Google Scholar]
  7. HALLANDER H. O. FRACTIONATION OF STAPHYLOCOCCAL TOXINS BY GEL-FILTRATION. Acta Pathol Microbiol Scand. 1963;59:543–552. doi: 10.1111/j.1699-0463.1963.tb01258.x. [DOI] [PubMed] [Google Scholar]
  8. HIRSCH J., AHRENS E. H., Jr The separation of complex lipide mixtures by the use of silicic acid chromatography. J Biol Chem. 1958 Aug;233(2):311–20. [PubMed] [Google Scholar]
  9. LOSOWSKY M. S., JONES D. P., DAVIDSON C. S., LIEBER C. S. STUDIES OF ALCOHOLIC HYPERLIPEMIA AND ITS MECHANISM. Am J Med. 1963 Dec;35:794–803. doi: 10.1016/0002-9343(63)90241-9. [DOI] [PubMed] [Google Scholar]
  10. NELSON W. L., BUTOW R. A., CIACCIO E. I. Oxidative phosphorylation in guinea pig mammary gland mitochondria during various functional states. Arch Biochem Biophys. 1962 Mar;96:500–505. doi: 10.1016/0003-9861(62)90327-2. [DOI] [PubMed] [Google Scholar]
  11. SHAH D. B., WILSON J. B. EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. I. NATURE OF THE SUBSTRATE AND ENZYME INVOLVED IN THE EGG YOLK OPACITY REACTION. J Bacteriol. 1963 Mar;85:516–521. doi: 10.1128/jb.85.3.516-521.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SLEIN M. W., LOGAN G. F., Jr Partial purification and properties of two phospholipases of Bacillus cereus. J Bacteriol. 1963 Feb;85:369–381. doi: 10.1128/jb.85.2.369-381.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WADDELL W. J. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med. 1956 Aug;48(2):311–314. [PubMed] [Google Scholar]
  14. WELD J. T., KEAN B. H., O'LEARY W. M. Production of octadecenoic acid in plasma by Staphylococcus aureus. Proc Soc Exp Biol Med. 1963 Feb;112:448–451. doi: 10.3181/00379727-112-28073. [DOI] [PubMed] [Google Scholar]
  15. WELD J. T., O'LEARY W. M. EVENTS ASSOCIATED WITH DEVELOPMENT OF LIPID PLAQUES ON PLASMA AGAR. Nature. 1963 Aug 3;199:510–511. doi: 10.1038/199510a0. [DOI] [PubMed] [Google Scholar]
  16. WILLIS A. T., TURNER G. C. Staphylococcal lipolysis and pigmentation. J Pathol Bacteriol. 1962 Oct;84:337–347. doi: 10.1002/path.1700840208. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES