Abstract
Bragg, P. D. (University of British Columbia, Vancouver, B.C., Canada), and W. J. Polglase. Enzymatic activities of streptomycin-dependent Escherichia coli in relation to valine formation. J. Bacteriol. 88:1399–1402. 1964.—The activities of several enzymes were compared in antibiotic-depleted and antibiotic-supplemented streptomycin-dependent Escherichia coli. Depleted cells were somewhat lower than supplemented cells in several oxidase activities. Isocitric dehydrogenase was very much lower in depleted cells than in supplemented cells. The lactic dehydrogenase activity of depleted and supplemented cells was similar. The balance of enzymatic activities in depleted and supplemented cells was thus found to correlate well with the observed extracellular products. Thus, depleted cells excreted lactic acid and were deficient in oxidase activity (although normal in lactic dehydrogenase activity), whereas supplemented cells excreted valine and were rich in the reduced nicotinamide adenine dinucleotide phosphate-producing enzyme, isocitric dehydrogenase. Since antibiotic-depleted cells were not deficient in lactic dehydrogenase, it appeared probable that the failure of depleted cells to metabolize lactate was related to a deficiency in the electron-transport system.
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- BODE F. Eine Vereinfachung und Verbesserung der Methode zur quantitativen Bestimmung von Aminosäuren und Peptiden mittels des Ninhydrin-Kupferkomplexes. Biochem Z. 1955;326(6):433–435. [PubMed] [Google Scholar]
- BRAGG P. D., POLGLASE W. J. ELECTRON-TRANSPORT COMPONENTS OF STREPTOMYCIN-DEPENDENT ESCHERICHIA COLI. J Bacteriol. 1963 Sep;86:544–547. doi: 10.1128/jb.86.3.544-547.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRAGG P. D., POLGLASE W. J. Extracellular metabolites of streptomycin mutants of Escherichia coli. J Bacteriol. 1962 Aug;84:370–374. doi: 10.1128/jb.84.2.370-374.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRAGG P. D., POLGLASE W. J. FORMATION OF VALINE BY STREPTOMYCIN-DEPENDENT ESCHERICHIA COLI. J Bacteriol. 1964 Oct;88:1006–1009. doi: 10.1128/jb.88.4.1006-1009.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRAGG P. D., REEVES R. E. Pathways of glucose dissimilation in Laredo strain of Entamoeba histolytica. Exp Parasitol. 1962 Oct;12:393–400. doi: 10.1016/0014-4894(62)90050-4. [DOI] [PubMed] [Google Scholar]
- ENGELBERG H., ARTMAN M. Studies on streptomycin dependent bacteria: effect of growth in limiting amounts of streptomycin on respiration and fermentation of a streptomycin dependent mutant of Escherichia coli. Biochim Biophys Acta. 1961 Mar 4;47:553–560. doi: 10.1016/0006-3002(61)90550-9. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- SCHAEFFER P. Recherches sur le métabolisme bactérien des cytochromes et des porphyrines. IV. Effets de la carence en streptomycine sur des mutants bactériens streptomycino-exigeants. Biochim Biophys Acta. 1952 Nov;9(5):563–571. doi: 10.1016/0006-3002(52)90207-2. [DOI] [PubMed] [Google Scholar]
- SPOTTS C. R. Physiological and biochemical studies on streptomycin dependence in Escherichia coli. J Gen Microbiol. 1962 Jun;28:347–365. doi: 10.1099/00221287-28-2-347. [DOI] [PubMed] [Google Scholar]
- SPOTTS C. R., STANIER R. Y. Mechanism of streptomycin action on bacteria: a unitary hypothesis. Nature. 1961 Nov 18;192:633–637. doi: 10.1038/192633a0. [DOI] [PubMed] [Google Scholar]
- TIRUNARAYANAN M. O., VISCHER W. A., RENNER U. Streptomycin and amino acid metabolism of bacteria. Antibiot Chemother (Northfield) 1962 Feb;12:117–122. [PubMed] [Google Scholar]