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. 1964 Nov;88(5):1459–1466. doi: 10.1128/jb.88.5.1459-1466.1964

ELECTRON MICROSCOPY OF A FISSION YEAST, SCHIZOSACCHAROMYCES POMBE

Norman Maclean a,1
PMCID: PMC277430  PMID: 14234806

Abstract

Maclean, Norman (University of Edinburgh, Edinburgh, Scotland). Electron microscopy of a fission yeast, Schizosaccharomyces pombe. J. Bacteriol. 88:1459–1466. 1964.—The structure of the fission yeast, Schizosaccharomyces pombe, was studied electron microscopically, with potassium permanganate and osmium tetroxide as fixatives. The cell was found to be bounded by a cell wall, 1,000 to 2,000 A thick, and a cell membrane. A layer of material was found between the cell membrane and the wall. The central nucleus, 2 to 3 μ in diameter, was bounded by a nuclear membrane, seen in some pictures to be double. Osmium tetroxide fixation revealed a granular body within the nucleus, identified as a nucleolus. Cytoplasmic structures included numerous vacuoles (probably normally containing lipid), a number of membranes and vesicles (which may represent a poorly organized mitochondrial system), and numerous granules (probably representing ribosomes).

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Selected References

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  1. CONTI S. F., NAYLOR H. B. Electron microscopy of ultrathin sections of Schizosaccharomyces octosporus. III. Ascosporogenesis, ascospore structure, and germination. J Bacteriol. 1960 Mar;79:417–425. doi: 10.1128/jb.79.3.417-425.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EDWARDS M. R., HAZEN E. L., EDWARDS G. A. The fine structure of the yeast-like cells of Histoplasma in culture. J Gen Microbiol. 1959 Jun;20(3):496–503. doi: 10.1099/00221287-20-3-496. [DOI] [PubMed] [Google Scholar]
  3. GANESAN A. T., SWAMINATHAN M. S. Staining the nucleus in yeasts. Stain Technol. 1958 May;33(3):115–121. doi: 10.3109/10520295809111834. [DOI] [PubMed] [Google Scholar]
  4. GLAUERT A. M., HOPWOOD D. A. The fine structure of Streptomyces coelicolor. I. The cytoplasmic membrane system. J Biophys Biochem Cytol. 1960 Jun;7:479–488. doi: 10.1083/jcb.7.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HASHIMOTO T., CONTI S. F., NAYLOR H. B. Studies of the fine structure of microorganisms. IV. Observations on budding Saccharomyces cerevisiae by light and electron microscopy. J Bacteriol. 1959 Mar;77(3):344–354. doi: 10.1128/jb.77.3.344-354.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HIRANO T., LINDEGREN C. C. Electron microscopy of mitochondrial changes in Saccharomyces. J Ultrastruct Res. 1963 Apr;8:322–326. doi: 10.1016/s0022-5320(63)90010-8. [DOI] [PubMed] [Google Scholar]
  7. HIRANO T. The fine structure of the nuclear apparatus in Saccharomyces. J Ultrastruct Res. 1962 Oct;7:201–209. doi: 10.1016/s0022-5320(62)90018-7. [DOI] [PubMed] [Google Scholar]
  8. KOEHLER J. K. The nuclear envelope of Saccharomyces cerevisiae. J Ultrastruct Res. 1962 May;6:432–436. doi: 10.1016/s0022-5320(62)80046-x. [DOI] [PubMed] [Google Scholar]
  9. LINDEGREN C. C. Nucleoprotein layer of the yeast cell. Nature. 1963 Jun 29;198:1325–1326. doi: 10.1038/1981325a0. [DOI] [PubMed] [Google Scholar]
  10. LINNANE A. W., VITOLS E., NOWLAND P. G. Studies on the origin of yeast mitochondria. J Cell Biol. 1962 May;13:345–350. doi: 10.1083/jcb.13.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MUNDKUR B. Electron microscopical studies of frozendried yeast. II. The nature of basophile particles and vesicular nuclei in Saccharomyces. Exp Cell Res. 1961 Oct;25:1–23. doi: 10.1016/0014-4827(61)90303-2. [DOI] [PubMed] [Google Scholar]
  12. ROBERTSON J. D. The ultrastructure of cell membranes and their derivatives. Biochem Soc Symp. 1959;16:3–43. [PubMed] [Google Scholar]
  13. RUSTAD R. C. Identification of the nucleus of a fission yeast with fluorescent dyes. Exp Cell Res. 1958 Oct;15(2):444–446. doi: 10.1016/0014-4827(58)90054-5. [DOI] [PubMed] [Google Scholar]
  14. SCHOPFER W. H., WUSTENFELD D., TURIAN G. [Nuclear division in Schizosaccharomyces pombe]. Arch Mikrobiol. 1963;45:304–313. doi: 10.1007/BF00405990. [DOI] [PubMed] [Google Scholar]
  15. VITOLS E., NORTH R. J., LINNANE A. W. Studies on the oxidative metabolism of Saccharomyces cerevisiae. I. Observations on the fine structure of the yeast cell. J Biophys Biochem Cytol. 1961 Mar;9:689–699. doi: 10.1083/jcb.9.3.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WYSS O., NEUMNN M. G., SOCOLOFSKY M. D. Development and germination of the Azotobacter cyst. J Biophys Biochem Cytol. 1961 Aug;10:555–565. doi: 10.1083/jcb.10.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. YOTSUYANAGI Y. [Electron-microscopic demonstration of chromosomes in yeasts by means of specific staining]. C R Hebd Seances Acad Sci. 1960 Feb 22;250:1522–1524. [PubMed] [Google Scholar]
  18. YOTSUYANAGI Y. [Study of yeast mitochondria. II. Mitochondria of respiration-deficient mutants]. J Ultrastruct Res. 1962 Aug;7:141–158. doi: 10.1016/s0022-5320(62)80032-x. [DOI] [PubMed] [Google Scholar]

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